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Regeneración de plantas via embriogénesis somática en Phaseolus acutifolius A. Gray
Resumen
Para desarrollar un sistema de transformación genética un requisito fundamental es contar con un protocolo de regeneración de plantas. En el trabajo se investigó la capacidad de Phaseolus acutifolius para regenerar plantas a partir de callos con estructuras embriogénicas. Se utilizaron dos tipos de explantes para la formación de callos en un medio de cultivo que contenía thidiazuron y ácido indol-acético. Los ejes embrionarios mostraron una mejor capacidad que los cotiledones para la formación de embriones somáticos. La luz solar resultó ser la más favorable para el desarrollo de las plantas in vitro. En estas condiciones se alcanzó el 32% de los embriones somáticos con germinación completa. Estos resultados indicanron que la formación de embriones somáticos y su germinación en Phaseolus acutifolius dependieron del explante, el medio de cultivo y las condiciones de iluminación. Las plantas germinadas in vitro, pudieron ser aclimatizadas en el invernadero.
Palabras clave: cultivo de tejidos, formación de callos, frijol, regeneración de plantas
Abbreviations: BAP 6- Benzylaminopurine, TDZ Thidiazuron, IAA indole-3-acetic acid, GA3 gibberellic acid, AgNO3 silver nitrate
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