Empleo de métodos biotecnológicos para la propagación de ñame
Resumen
En el cultivo del ñame, la propagación convencional presenta bajos índices de multiplicación. Por ello, ha sido necesario el uso de métodos biotecnológicos para garantizar la disponibilidad de material vegetal de plantación. La producción de plantas in vitro y microtubérculos permite la obtener semillas con calidad fisiológica y sanitaria, pero los protocolos desarrollados han presentado limitaciones para su empleo comercial. Los microtubérculos presentan numerosas ventajas en comparación con las plantas in vitro, pero estos se han obtenido en medios de cultivo en estado semisólido y se han caracterizado por una masa fresca promedio inferior a 0.5 g que ha limitado su utilización. El empleo de sistemas de cultivo semi-automatizado puede constituir una alternativa de gran interés para la formación de microtubérculos de ñame que permita emplearlos como material vegetal de plantación directo en campo. El presente trabajo presenta una revisión de literatura científica sobre los aspectos anteriores.
Palabras clave: Dioscorea spp., material vegetal de plantación, microtubérculos
Referencias
Acha IA, Shiwashi H, Asiedu R, Akoroda MO (2004) Effect of auxins on root development in yam (Dioscorea rotundata) vine. Tropical Science 44 (2): 113-119
Akita M, Takayama S (1994) Induction and development of potato tubers in jar fermentor. Plant Cell Tissue and Organ Culture 36: 177-182
Akita M y Ohta Y (1998) A simple method for mass propagation of potato (Solanum tuberosum L.) using a bioreactor without forced aeration. Plant Cell Reports 18: 284-287
Amusa N, Adegbite A, Amuhammeda S, Baiyewu RA (2003) Yam diseases and its management in Nigeria. African Journal of Biotechnology 2 (12): 497-502
Balogun MO, Ng SYC, Shiwachi H, Ng NQ, Fawole I (2004) Comparative effects of explant sources and genotypes on microtuberization in yams (Dioscorea spp.). Tropical Science 44 (4): 196-200
Balogun, MO (2005) Development of microtuber production and dormancy control protocols for yam (Dioscorea spp.) germplasm conservation. PhD Thesis. University of Ibadan, Nigeria.
Balogun MO, Fawole I, Ng SYC, Ng Q, Shiwachi H, Kikuno H (2006) Interaction among cultural factors in microtuberization of white yam (Dioscorea rotundata Poir). Tropical Science 46 (1): 55-59
Balogun MO (2009) Microtubers in yam germplasm conservation and propagation: The status, the prospects and the constraints. Biotechnology and Molecular Biology Reviews 4 (1): 1-10
Bazabakana R, Wattiez R, Baucher M, Diallo B, Jaziri M (2003) Effect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata (L.). Plant Growth Regulation 40: 229-237
Berthouly M, Etienne H (2005) Temporary immersion systems: a new concept for use liquid medium in mass propagation. En: Hvoslef-Eide A K y Preil W (Eds). Liquid Culture Systems or in vitro Plant Propagation. pp. 165-195. Springer. Dordrecht
Borges M, Meneses S, Aguilera N, Vázquez J (2004) Regeneration and multiplication of Dioscorea alata germplasm maintained in vitro. Plant Cell Tissue and Organ Culture 76 (1): 87-89
Cabrera J, Kosky RG, Rayas C, De Feria M, López T, Basail P, Medero V (2009) Protocolo para la formación de microtubérculos de ñame (Dioscorea alata L.) en sistema de inmersión temporal. Revista Colombiana de Biotecnología XI (2): 19-30
Chen FY, Wang D, Gao X, Wang L (2007) The effect of plant growth regulators and sucrose on the micropropagation of Dioscorea nipponica makino. Plant Growth Regulation 26: 38-45
Curtis WR (2005) Application of bioreactor design principles to plant Micropropagation. En: AK Hvoslef-Eide y W Preil (Eds.). Liquid Culture Systems for in vitro Plant Propagation, pp. 21–40. Springer. Dordrecht
Escalona M, Samaon G, Borroto C, Desjardins Y (2003) Physiology of effects of termporary immersion bioreactors on micropropagated pineapple plantlets. In vitro Cell. Dev. Biol. Plant 39: 651-656
Escalona, M (2006) Temporary immersion beats traditional techniques on all fronts. Prophyta annual: 48-50
Escalona M, Aragón C, Capote I, Pina D, Cejas I, Rodríguez R, Cañal M, Sandoval J, Roels S, Debergh P, Desjardins Y, González-Olmedo J (2007) Physiology of effects of temporary immersion bioreactor (TIB) on micropropagated plantlets. Acta Hort. 748: 95-101
Filipia R, Algora P (1996) Métodos de reproducción acelerada de semilla de ñame. En: XXX Aniversario del INIVIT. Instituto de Investigaciones en Viandas Tropicales (INIVIT), Santo Domingo, Cuba
Forsyth C, Van Staden J (1984) Tuberization of Dioscorea bulbifera stem nodes in culture. J. Plant Physiol 115: 79-83
García M, Escalona M, Meneses S (2004) Efecto de la concentración de sacarosa y de reguladores de crecimiento en la tuberización in vitro de ñame. Biotecnología Vegetal 4 (4): 184-187
González JE (2006) Diagnóstico de enfermedades virales pertenecientes al género de los Potivirus en los geneotipos de ñame ‘Pacala Duclos’ (Dioscorea
alata L.) y ñame de Guinea (Dioscorea rotundata Poir.). Aplicaciones de la corriente eléctrica. Tesis de Maestría. Universidad Central de Las Villas, Instituto de Biotecnología de las Plantas. Cuba. 85 p.
Hahn EJ, Paek KY (2005) Multiplication of Chrysanthemum shoots in bioreactors as affected by culture method and inoculation density of single node stems. En: AK Hvoslef-Eide y W Preil (Eds.), Liquid Culture Systems for in vitro Plant Propagation, pp. 143–153. Springer. Dordrecht
Hempfling T, Preil W (2005) Application of a temporary immersion system in mass propagation of Phalaenopsis. En: AK Hvoslef-Eide y W Preil (Eds.), Liquid Culture Systems for in vitro Plant Propagation, pp. 231–242. Springer. Dordrecht
Ijoyah MO, Aba J, Ugannyan S (2006) The effects of seedbed types on yam minisetts yield: A case study of Ushongo local government area of Benue state of Nigeria. African Journal of Biotechnology 5 (22): 2086-2091
Jackson MB (2005) Aeration stress in plant tissue cultures. En: AK Hvoslef-Eide y W. Preil (Eds.), Liquid Culture Systems for in vitro Plant Propagation, pp. 459–473. Springer. Dordrecht
Janssenns M (2001) Yam. En: Raemaerkers R (Eds). Crop production in tropical Africa. pp. 229-245. Falta editorial
Jasik J, Mantell SH (2000) Effect of jasmonic acid and its methylester on in vitro microtuberization of three food yam (Dioscorea) species. Plant Cell Reports 19: 863-867
Jean M, Cappadocia M (1992) Effects of some growth regulators on in vitro tuberization in Dioscorea alata L. ´Brazo fuerte´ and D. abyssinica Hoch. Plant Cell Reports 11: 34-38
Jiménez E, Pérez N, De Feria M, Barbón R, Capote A, Chávez M, Quiala E, Pérez JC (1999) Improved production of potato microtubers using a temporary immersion system. Plant Cell Tissue and Organ Culture 59: 19-23
Jiménez E (2005) Mass propagation of tropical crops in temporary immersion systems. En: Hvoslef-Eide A K y Preil W (Eds). Liquid Culture Systems or in vitro Plant Propagation, pp. 197-211. Springer. Dordrecht
Kawakami J, Iwama K, Hasegawa T, Jitsuyama Y (2003) Growth and Yield o f Potato Plants Grown from Microtubers in Fields. Amer. J. of Potato Res 80: 371-378
Kawakami J, Iwama K, Yutaka K, Jitsuyama Y (2005) Effects of planting date on the growth and yield of
two potato cultivars grown from microtubers and conventional seed tubers. Plant Production Science 8 (1): 74-78
Kikuno H, Onjo M, Kusigemati K, Hayashi M (2002) A relationship between the initiation of tuber enlargement and endogenous plant hormones in water yam (Dioscorea alata L.). Jpn. J. Trop. Agric. 46 (1): 39-46
Kim EK, Hahn EJ, Murthy HN, Paek KY (2005) Enhanced shoot and bulblet proliferation of garlic (Allium sativum L.) in bioreactor systems. Journal of Horticulture Science & Biotechnology 79 (5): 818-822
Kozai T, Kubota C (2005) Unit and terminology use for the studies of photoautotrophic micropropation. En: T Kozai, F Afreen, SMA Zobayed (Eds). Photoautotrophic (sugar-free medium) micropropagation as a new propagation and transplant production system, pp. 6-17. Springer.Dordrech
Krikorian A (1991) Medios de cultivos: Generalización, composición y preparación. En: Roca, W y L Mroginski (Eds). Cultivo de tejidos en la agricultura. Fundamentos y aplicaciones, pp. 41-79. CIAP, Cali
Labrada M, Millán A, Cruz G (1997) Influencia de los brasinosteroides en la tuberización in vitro del ñame (Dioscorea alata L.). Centro Agrícola 20 (1): 80-81
Lebas BSM (2002) Diversity of viroses infesting Dioscorea species in the south pacific. A thesis submitted in partial fulfillment of the requirements of the University of Greenwich for the Degree of Doctor of Philosophy (PhD). The University of Greenwich Natural Resources Institute. 123 p.
Lowell, L, Dilworth F, Omoruyi P, Helen N (2007) In vitro availability of some essential minerals in commonly eaten processed and unprocessed Caribbean tuber crops. Biometals 20 (1): 37-42
Malaurie, B, Pungu O, Trouslot M (1995) Effect of growth regulators concentrations on morphological development of meristem tips in Dioscorea cayenensis-D. rotundata complex and D. praehensilis. Plant Cell Tissue and Organs and Culture 41: 229-235
Mantell S, Hugo A (1989) Effect of photoperiod mineral medium strength, inorganic ammonium, sucrose and cytokinina on root, shoot and microtuber development in shoot cultures of Dioscorea alata L. and D. bulbifera L. yams. Plant Cell Tissue and Organ Culture 16: 23-39
Mbanaso, ENA, Chukwu LI, Opara MUA (2007) In vitro basal and nodal microtuberization in yam shoot cultures (Discorea rotundata Poir, cv. Obiaoturugo) under nutritional stress conditions. African Journal of Biotechnology 6 (21): 2444-2446
McAlister, B, Finnie J, Watt MP y Blakeway F (2005) Use of the temporary immersion bioreactor system (RITA®) for production of commercial Eucalyptus clones in Mondi Forests (SA). En: AK Hvoslef-Eide y W Preil (Eds.). Liquid Culture Systems for in vitro Plant Propagation, pp. 425–442. Springer. Dordrecht
Medero, V, Del Sol L, García M (1999) Metodología para la propagación del clon de ñame ‘Blanco o Pelú’. Resúmenes de BioCat 99, Granma Cuba 5-7 de Octubre pp. 12
Mehrotra, S, Manoj G, Arun K, Bhartendu M (2007) Efficiency of liquid culture systems over conventional micropropagation: A progress towards commercialization. African Journal of Biotechnology 6 (13): 1484-1492
Milián, M, Girado Y, Beovides Y, Lago M, Hernández J, Ruiz E (2005) The germplasm bank an alternative to preserve the biodiversity. Revista Internacional Agrisost 12 (1): 20-28
Mitchell, SA, Asemota NH, Mohammad HA (1995) Effects of explants source, culture medium strength and growth regulators on the in vitro propagation of three Jamaican yams (Dioscorea cayenensis, D. trifida and D. rotundata). J Sci Food Agric. 67 (4): 173-180
Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol. Plant. 15: 473-497
Ng SYC (1992) Micropropagation of white yam (Dioscorea rotundata Poir.) Biotechnology in Agriculture and Forestry 19: 136-159
Ng SYC, Ng NQ (1997) Germplasm conservation in food yams (Dioscorea spp.): Constraints, Application and Future prospects. En: Razdan MK, Cocking EC. U.A (Eds) conservation of plant Genetic resources in vitro. Volume 1: General Aspects, pp. 257-286. Science publishers Inc. New York
Ovono PO, Kevers C, Dommes J (2007) Axillary proliferation and tuberization of Dioscorea cayenensis-D. rotundata complex. Plant Cell Tissue and Organ Culture 91: 107-114
Pathirana, R, Harris J, Marian J, McKenzie M (2008) A comparison of microtubers and field-grown tubers of potato (Solanum tuberosum L.) for hexoses, sucrose and their ratios following postharvest cold storage. Postharvest Biology and Technology 49 (1): 180-184
Paulo E, Ribeiro R (2002) Growth and carbohydrate changes in shoot cultures of Dioscorea species as influenced by photoperiod, exogenous sucrose and cytokinin concentrations. Plant Cell Tissue and Organ Culture 70: 241-249
Perea M (2001) Contribución de la biotecnología al desarrollo sostenible del cultivo del ñame. En: Perea, M. (Eds). Biotecnología Agrícola. pp. 289-301. Bogotá, D.C.
Pérez, N (2001) Producción de microtubérculos de papa (Solanum tuberosum L.) en Sistemas de Inmersión Temporal. Tesis de Maestría. Universidad Central de Las Villas, Instituto de Biotecnología de las Plantas. Cuba. 85 p.
Piao XC, Chakrabarty D, Hahn EJ, Paek KY (2003) A simple method for mass production of potato microtubers using a bioreactor system. Current Science 84 (8): 1129-1132
Preil W (2005) General introduction: a personal reflection on the use of liquid media for in vitro culture. En: AK Hvoslef-Eide y W Preil (Eds.). Liquid Culture Systems for in vitro Plant Propagation, pp. 1–18. Springer. Dordrecht
Ranalli P (1997) Innovative propagation methods multiplication programmes in seed tuber. Potato Research 40: 439-45
Read, P (2007) Micropropagation: Past, present and future. Acta Hort. 748: 17-28
Rodríguez W (2000) Botánica, domesticación y fisiología del cultivo del ñame (Dioscorea alala). Agronomía Mesoamericana 11 (2): 133-152
Rodríguez S (2004) Situación actual y perspectivas de los cultivos varios. Informe a la Asamblea Nacional del Poder Popular. Ministerio de la Agricultura. Ciudad de La Habana, 29 de Junio del 2004
Rodríguez S (2006) Evaluación y recomendación de clones de boniato, yuca, ñame, plátanos y bananos resistentes o tolerantes a los factores adversos de la producción (FAP) y su manejo integrado. Informe final, Programa Nacional Científico. p. 67
Saborio F, Torres SA, Gómez L (2002) Development of a clean-planting-material production system on tropical root and tuber crops, using in vitro propagated plants. Scientia Horticulture 641: 495-501
Salazar RD, Beltran JDH (2003) Microtuberización en ñame (Dioscorea alata L.) var. ‘Pico de botella’. Revista Colombiana de Biotecnología 4 (2): 27-32
Salazar D, Hoyos S (2007) Multiplicación y tuberización in vitro de ñame (Dioscorea alata L.) en sistema de inmersión temporal. Rev. Fac. Nal. Agr. Medellín 60 (2): 3907-3921
Sarkar D (2008) The signal transductions pathways controlling in plant tuberizations in potato: an emerging synthesis. Plant Cell Reports 27: 1-8
Savangikar VA, Savangikar C, Daga RS, Pathak S (2005) Potentials for cost reduction in a new model of commercial micropropagation. En: AK Hvoslef-Eide y W Preil (Eds.). Liquid Culture Systems for in vitro Plant Propagation, pp. 403–414. Springer. Dordrecht
Scarcelli N, Tostain S, Mariac C, Agbangla C, Da O, Berthaud J, Pham J (2006) Genetic nature of yams (Dioscorea sp.) domesticated by farmers in Benin (West Africa). Genetic Resources and Crop Evolution 53: 121–130
Scott G, Rosegrant M, Ringler C (2006) Roots and tubers for the 21st Century: Trends, projections, and policy options. Food, Agriculture and the Environment Discussion 31. Washington, DC: International Food Policy Research Institute (IFPRI) and International Potato Center (CIP)
Takayama S, Misawa M (1981) Mass propagation of Begonia hiemalis plantlets by shake culture. Plant Cell Phisiol. 22: 462-467
Takayama S, Akita M (2005) Practical aspects of bioreactor application in mass propagation of plants. En: AK Hvoslef-Eide y W Preil (Eds.). Liquid Culture Systems for in vitro Plant Propagation, pp. 61–78. Springer. Dordrecht
Tamiru, M, Becker HC, Maass BL (2008) Diversity, distribution and management of yam landraces (Dioscorea spp.) in Southern Ethiopia. Genet Resour Crop Evol. 55: 115-131
Teisson C, Alvard D (1999) In vitro production of potato microtubers in liquid medium using temporary immersion. Potato Research 42: 499-504
Teisson, C, Alvard D, Berthouly M, Cote F, Escalant J, Etienne H, Lartand M (1996) Simple apparatus to perform plant tissue culture by temporary immersion. Acta Hort. 440: 521-526
Tisserat B, Silman R (2000) Interaction of cultures vessels, media volume, culture density, and carbon dioxide levels on lettuce and spearmint shoot growth in vitro. Plant Cell Reports 19: 464-471
Vaillant V, Bade P, Constant C (2005) Photoperiod affects the growth and development of yam plantlets obtained by in vitro propagation. Biologia Plantarum 49 (3): 355-359
Wilson JE (1989) Rapid multiplication of yams (Dioscorea spp.). Institute for Research, Extension and Training in Agriculture. IRETA Publication
Yu W, Joyce P, Cameron D, McCown B (2000) Sucrose utilization during potato microtuber growth in bioreactors. Plant Cell Reports 19: 407-413
Ziv M (2000) Bioreactor technology for plant micropropagation. Horticultural reviews 24, Edited by Jules Janick
Ziv M (2005) Simple bioreactor for mass propagation of plants. En: Hvoslef-Eide A K y Preil W (Eds). Liquid Culture Systems or in vitro Plant Propagation, pp. 79-93. Springer. Dordrecht
Copyright (c) 2016 Biotecnología Vegetal
Biotecnología Vegetal eISSN 2074-8647, RNPS: 2154. ISSN 1609-1841, RNPS: 0397 Editada por: Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las Villas. Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba CP 54 830 Tel: 53 42200124, e-mail: info@ibp.co.cu
Biotecnología Vegetal está bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.