Comparación de fragmentos de genes que codifican para proteínas ribosomales de caña de azúcar
Resumen
Secuencias parciales de genes de caña de azúcar obtenidos como resultado de una biblioteca sustractiva, fueron comparadas con las existentes en bases de datos, mediante el algoritmo BLAST. Durante la búsqueda de homología se identificaron cinco genes que codifican para proteínas ribosomales, tanto de cloroplastos como citoplasmáticas. La mayor homología obtenida entre las secuencias identificadas como genes codificantes de proteínas ribosomales de caña de azúcar fue con el genoma de maíz.
Palabras clave: biblioteca sustractiva, dominios consensus, Saccharum spp.
Referencias
Vegetal 3: 179 –181
Barakat, A, Szick-Miranda, K, Chang, I, Guyot, R, Blanc, G,
Teixeira-Gomes, A P, Cloeckaert, A, Zygmunt, MS (2000) Cooke, R, Delseny, M, Bailey-Serres, J (2001) The
Characterization of heat, oxidative, and acid stress Organization of Cytoplasmic Ribosomal Protein Genes in the
responses in Brucella melitensis. Infection and Immunity Arabidopsis Genome. Plant Physiology 127: 398-415
: 2954–2961
Brosche, M, Strid, A (1999). The mRNA-binding ribosomal
Volarevic, S, Stewart, MJ, Ledermann, B, Zilberman, F, protein S26 as a molecular marker in plants: molecular
Terracciano, L, Montini, E, Grompe, M, Kozma SC, Thomas, G cloning, sequencing and differential gene expression during
(2000) Proliferation, but not growth, blocked by conditional environmental stress. Biochimica et Biophysica Acta 1445:
deletion of 40S ribosomal protein S6. Science 288: 2045-2047 342-344
Gantt, JS, Key, JL (1985) Coordinate expression of ribosomal protein mRNAs following auxin treatment of soybean hypocotyls. J. Biol. Chem. 260: 6175-8
Garo, J, Kim, SR, Chung, YY, Lee, JM, An, G (1994) Developmental and environmental regulation of two ribosomal protein genes in tobacco. Plant Mol. Biol. 25: 761-77
Ito, T, Kim, GT, Shinozaki, K (2000) Disruption of an Arabidopsis cytoplasmatic ribosomal protein S13-homologous gene by transposon-mediated mutagenesis causes aberrant growth and development. Plant Journal 22: 257-64
Lambertsson, A (1998) The minute genes in Drosophila and their molecular functions. Adv. Genet. 38: 69-134
Liu, X, Baird, WV (2003) Differential expression of genes regulated in response to drought- or salinity-stress from Helianthus annuus L. (sunflower). Crop Science 43: 678–687
Ludwig, A, Tenhaken R (2001) Suppression of the ribosomal L2 gene reveals a novel mechanism for stress adaptation in soybean. Planta 212: 792–798
Luo, M, Kong X, Huo N, Zhou R, Jizeng, JIA (2002) Gene expression profiling related to powdery mildew resistance in wheat with the method of suppression subtractive hybridization. Chinese Science Bulletin 47 (23): 1990-1994 23
Oloriz, M I, Rojas, L, Gil, V, Jiménez E (2002) Librería sustractiva obtenida de un mutante de la variedad B 4362 con resistencia a la roya de la caña de azúcar. Biotecnología
Watt, DA (2003) Aluminium-responsive genes in sugarcane: identification and analysis of expression under oxidative stress. Journal of Experimental Botany 385: 1163-1174
Williams, ME, Sussex, LM (1995) Developmental regulation of ribosomal protein L16 genes in Arabidopsis thaliana. Plant Journal 8: 65-76
Wittmann-Leibold, B, Kopke, AKE, Arndt, E, Kromer, W, Hatakeyama, T, Wittmann, H-G (1990) Sequence comparison and evolution of ribosomal proteins and their genes. En: WE Hill, A Dahlberg, RE Garrett, PB Moore, D Schlessinger, JR Warner(eds), The Ribosome, Structure, Function and Evolution, pp 598-616. American Society of Microbiologists, Washington
Wool, IG (1996). Extraribosomal functions of ribosomal proteins. Trends in Biochemistry 21: 164–165
Yamaguchi, K, von Knoblauch, K , Subramanian, AR (2000) The plastid ribosomal proteins: identification of all the proteins in the 30 S subunit of an organelle ribosome (chloroplast). J. Biol. Chem. 275: 28455-28465
Yamaguchi, K, Prieto, S, Beligni, MV, Haynes, PA, McDonald, HW, Yates, JR, Mayfield, SP (2003) Proteomic Characterization of the Chlamydomonas reinhardtii Chloroplast Ribosome. The Journal of Biological Chemistry 278: 33774–33785
Zinn, AR, Ross, JL (1998) Turner syndrome and haploin sufficiency. Curr. Opin. Genet. Dev. 8: 322-327
Copyright (c) 2016 Biotecnología Vegetal

Biotecnología Vegetal eISSN 2074-8647, RNPS: 2154. ISSN 1609-1841, RNPS: 0397 Editada por: Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las Villas. Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba CP 54 830 Tel: 53 42200124, e-mail: info@ibp.co.cu
Biotecnología Vegetal está bajo una Licencia Creative Commons Atribución-NoComercial 4.0 Internacional.