Control de la persistencia de una cepa genéticamente modificada de Azorhizobium caulinodans en la rizosfera de Triticum aestivum L.

Jan Jacek Slaski, Daniel Joseph Archambault, John Vidmar, Carlos José Bécquer, Anthony Anyia

Resumen


Se llevó a cabo un protocolo para monitorear la persistencia y multiplicación de una cepa genéticamente modificada de la bacteria diazotrófica Azorhizobium caulinodans, la cual se usó para inocular trigo sembrado en condiciones de campo. El protocolo se utilizó para identificar una cepa productora de endogluconasa (egl), aislada del suelo inoculado. Se determinaron los límites de detección para los métodos de identificación BIOLOG® (2500 ufc/g-1), y PCR (108_1010ufc/g-1 de suelo). Ya que ninguna de las muestras evaluadas resultó positiva para la bacteria o el gen Egl1, se concluye que A. caulinodans ORS 571 pGV910-C1 no persistió, ni se multiplicó en ninguno de los experimentos de campo evaluados. Este resultado puede ser debido a las bajas temperaturas del suelo y a la competencia de microorganismos nativos, lo cual constituye un factor ambiental que obstaculiza el desarrollo de la bacteria diazotrófica en los sitios experimentales. Se recomienda la aplicación de diferentes metodologías de inoculación, así como el estudio de otros géneros de rizobios para la inoculación de trigo en futuros experimentos.

Palabras clave: diazotrófica, endogluconasa, identificación, ORS 571, trigo


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Referencias


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