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Efecto de la densidad de inóculo y la renovación de la atmósfera gaseosa en el cultivo de brotes de Digitalis purpurea L. en Sistemas de Inmersión Temporal
Resumen
Digitalis purpurea L. es una de las dos únicas especies de interés económico del género Digitalis. Su importancia radica en que constituye una de las principales fuentes de cardenólidos, fármacos irreemplazables en el tratamiento de la insuficiencia cardíaca. El presente trabajo se realizó con el objetivo de determinar el efecto de la densidad de inóculo y la renovación de la atmósfera gaseosa en sistemas de inmersión temporal (SIT) en Digitalis purpurea L. Para su cumplimiento, se estudiaron diferentes densidades de inóculo (6, 12, 18 explantes por SIT) en la producción de biomasa así como el uso de la ventilación forzada. La mayor producción de biomasa por SIT (104.03 gMF y 5.74 gMS) se obtuvo cuando se inocularon 12 explantes. Sin embargo, la menor densidad de inóculo produjo un efecto negativo en el desarrollo de los explantes y no hubo incremento de biomasa. La renovación de la atmósfera mediante la ventilación forzada indujo un mayor estrés oxidativo asociado a un aumento de la síntesis de digitoxina. Tanto la densidad de inóculo como la ventilación forzada son factores de gran importancia en el cultivo en sistemas de inmersión temporal ya sea para la producción de biomasa como para la obtención de metabolitos secundarios en plantas de interés.
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