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Caracterización molecular de variedades de caña de azúcar cultivadas en el estado de Tabasco, México
Resumen
El objetivo del presente trabajo fue caracterizar molecularmente la variabilidad genética en variedades de caña de azúcar (Saccharum spp.) cultivadas en el estado de Tabasco, México, mediante AFLP. Se utilizaron 12 variedades de caña de azúcar de las cuales se tomaron hojas jóvenes de plantas en campo. Después de realizar 12 combinaciones de cebadores, los resultados indicaron que la combinación E-ACC/M-CTA produjo fragmentos polimórficos de 72 a 1353 pb. El dendrograma reveló dos grupos distintos de Saccharum spp. y una variedad que se diferenció de ambos. El grupo I comprendió las variedades C 87-51, ATM 96-40, B 4362, Mex 69-290, Mex 57-1285 y Mex 91-130, que formaron un conglomerado y presentaron una similitud del 0.77%. El grupo II comprendió las variedades RD 75-11, Mex 79-431, SP 70-1284, Mex 59-32 y CP 72-2086 las cuales conformaron otro conglomerado con 0.70%. La variedad Mex 68-P-23 se diferenció de las otras 11 variedades.
Palabras clave: ADN, AFLP, marcadores moleculares
Referencias
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