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Aspectos relacionados con el ambiente físico y la caracterización molecular de la embriogénesis somática
Resumen
En el presente trabajo se realiza una breve reseña sobre la influencia del ambiente in vitro en el proceso morfogenético de la embriogénesis somática, específicamente el efecto que tienen determinados gases que se acumulan en la fase gaseosa de los frascos de cultivo como son el oxígeno, el dióxido de carbono y el etileno sobre la embriogénesis somática. Se hace referencia también a biomoléculas como las proteínas, isoenzimas, poliaminas y arabinogalactanoproteínas que están relacionadas con el proceso morfogenético de la embriogénesis somática.
Palabras clave: ambiente in vitro, Embriogénesis somática, embrión somático, marcadores moleculares
Referencias
Albrechtová, JTP, Cvikrová M, Eder J (1994) Differentiation and polyamines level in primary leaf explants from alfalfa cultured on embryogenic and nonembryogenic media. Bio Planten 36 (Supp.) Abstract 9th of Brno. Praga. Czech Rep. 3-8 July
Ammirato, PV (1987) Organizational events during somatic embryogenesis. En:Green, CE, Somers DA, Hackett WP y Biesboer DD (eds) Plant Tissue and Cell culture Alan R. Liss. Inc. pp. 57-81 New York
Ammirato, PV y Steward FC (1971) Some effects pf the ent viroment of the development of embryos from cultured free cell. Bot Gaz. 132: 149-158
Ammirato, PV (1983) Embryogenesis. S. En: Evans D A, Sharp W R, Ammirato P V, Yamada Y (eds.). Handbook of plant cell culture. Vol. 1: Techniques for propagation and breeding. pp. 182-123. Macmilland Publishing Co. New York, Collier Macmilland Publishers London
Archambault, J, Lavoie L, Williams RD, Chavarie C (1995) Nutritional aspects of Daucus carota somatic embryo cultures performed in bioreactors. En: Terzi, M (Ed.), Current Issues in Plant Molecular and Cellular Biology, pp. 681-687 Kluwer Academic Publisher, Dordrecht
Ashihara, H, Fujimura T, Komamine A (1981) Prymidine nucleotide byosinthesis during somatic embryogenesis in carrot cell suspension culture. Z. Pflanzenphysiol 104: 129-137
Auboiron, E, Carron MP, Michaux-Ferrière N (1990) Influence of atmospheric gases, particularly ethylene, on somatic embryogenesis of Hevea brasiliensis. Plant Cell Tiss. Org.Cult. 21: 31-37
Beranger-Novat, N, Bonneau L, Martin-Tangunin J, Monin J (1990) Poliamine metabolism-evolution and effects of its specific inhibitors with dormancy breaking in zygotic embryos of a woody species Euonymus europeaus L cultured in vitro. En: Flores HE, Arteca RN, Shannon JC (eds.) Plant Physiology, biosynthesis and molecular regulation of amino acids in plants. American Society of Plant Physiologists: pp. 363-364 Kluwer Academic Publisher, Dordrecht
Berros, B (1996) Ontogenia y caracterización molecular e histológica de la organogénesis-embriogénesis en avellano. Tesis Doctoral. Universidad de Oviedo. España
Biddington, NL (1992) The influence of ethylene in plant tissue culture. Plant Growth Reg. 11: 173-187
Buddendorf-Joosten, JMC y Woltering EJ (1994) Components of the gaseous enviroment and their effectts on plant growth and development in vitro. S. 165-190 En: Lumsden PJ, Nicholas J, Daves WJ (eds) Physiology, growth and development of plants in culture. Kluwer Academic Publishers Dordrecht, Boston, London
Cazzulino, D, Pedersen H, Chin CK (1991) Bioreactors and image analysis for scale-up and plant propagation. Cell Cult. Somatic Cell Genet. Plants 8: 147-177
Cevallos, AM (2000) Establecimiento de una metodología eficiente en el proceso de embriogénesis somática del cafeto (Coffea spp.), mediante el uso de marcadores morfofisiológicos y moleculares. Tesis de Doctorado. Univ. Agraria de La Habana
Chen, SJ y Kao CH (1997) Ammonium inhibited growth of suspension-cultured rice cells as affected by medium pH. Plant Growth Regul. 21: 1-6
Choi, JH, Liu L, Borkird C, Sung ZR (1987) Cloning of genes developmentally regulating during plant embryogenesis. Proc. Natl. Acad. Sci. USA. 84: 1906-1910
Cordewener, J, Booij H, Van der Zandat H, Van Engelen F, Van Kammer A, Vries S (1991) Tunicamycim-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes. Planta 184: 478-486
Coutos-Thevenot, P, Maes O, Jouennen T, Mauro MC, Boulay M, Deloire A, Guern J (1992) Extracellular protein patterns of grapevine cell suspensions in embryogenic and non-embryogenic situations. Plant Sci. 86: 137-145
Coutos-Thevenot, P, Maes O, Jouennen T, Mauro MC, Boulay M, Deloire A, Guern J (1992) Extracellular protein patterns of grapevine cell suspensions in embryogenic and non-embryogenic situations. Plant Sci. 86: 137-145
de Feria, M (2000) Empleo de bioreactores para la multiplicación y diferenciación de suspensiones celulares de Coffea arabica L. cv. Catimor 9722. Tesis presentada en opción al grado científico de Doctor en Ciencias Agrícolas. Instituto de Biotecnología de las Plantas. Villa Clara. 105 p.
de Vries, SC, Booij H, Meyerink P, Huisman G, Wilde HD, Thomas TL, Van Kammen A (1988) Adquisition of embryogenic potential in carrot cell-suspension cultures. Planta 176: 196-204
Denchev, PD, Kuklin AI, Scragg AH (1992) Somatic embryo production in bioreactors. J. Biotechnol. 48: 115-168
Ding L, Zhu JK (1997) A role for arabinogalactan-proteins in root epidermal cell expansion. Planta 203: 289- 294
Du, H, Clarke AE, Bacic A (1996) Arabinigalactan-protein: a class of extracelluler proteoglycans involved in plant growth and development. Trends Cell Biol 6: 411-414
Ducos JP, Zamarripa A, Eskes A, Pétiard V (1993) Production of somatic embryos of coffee in a bioreactor. En: 15éme Colloq. Sci. Int. Café. Montpellier, 6-11 June pp. 86-96. ASIC. Paris
Dure, L (1985) Embryogenesis and gene expression during seed formation. Plant Mol. Cell Biol. 2: 179-197
Egertsdotter, U, von Arnold S (1995) Important of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies) Physiologia Plantarum 93: 334-345
Esashi, Y, Ishizawa K (1989) Oxygen-independent ethylene action in cocklebur seed germination in relation to osmoregulation. En: Clijsters, (ed.). Biochemical and physiological aspects of ethilene production in flower and higher plants. Klumer Academic Publisher Dordrech
Everette, NP, Wach MJ, Ashworth DJ (1985) Biochemical markers of embryogenesis in tissue culture of the maize inbred B 73. Plant Sci. 41: 133-140
Gananapragasam S, Vasil IK. 1992. Cryoconservation of inmature embryos, embryogenic callus and cell suspension cultures of gramineous species. Plant Science 83: 205-215
Gepts, P (1994) Análisis molecular del proceso de domesticación en plantas: el ejemplodel frijol (Phaseolus vulgares). En: 11 Congreso Latinoamericano de Genética y XV Congreso de Fitogenética, Monterrey, Nueva León, México
Gupta PK, Timmis R, Pullman G, Yabncey M, Kreitinger M, Carlson W, Carpenter C (1991) Development of the embryogenic system for automated prooagation of forest trees. En: Vasil, I K (ed). Scale-up and automation in plant propagation. Cell culture and somatic cell genetic of plant. Vol. 8. pp. 75-93 Academic Press. Inc. San Diego
Harrel RC, Bieniek M, Hood CF,Munilla R, Cantliffe DJ (1994) Automated, in vitro harvest of somatic mebryos. Plant Cell Tiss. Org. Cult. 39: 171-183
Hatzapoulos P, Fong F, Sung ZR (1990) Abscisic acid regulation of DC8, a carrot embryogenic gene. Plant Physiol 94: 690-695
Hayashi M, Kosai T, Tateno M, Fujiwara K, Kitaya Y (1993) Effects of the lighting cycle on the growth and mophology of potato plantlets in vitro under Photomixotrophic culture conditions. Environ. Contorl Biol. 31(3): 169-175
Heo J, Kozai T (1999) Forced ventilation micropropagation system for enhancing photosynthesis, growth and development of sweetpotato plantlets. Environment Control in Biology 37 (1): 83-92
Heo, J y Kozai,T (1999) Forced ventilation micropropagation system for enhancing photosynthesis, growth and development of sweetpotato plantlets. Environment Control in Biology 37 (1): 83-92
Hergarty PK, Smart NJ, Scragg AH, Fowler MW (1986) The aeration of Cataranthus roseus L. G. Don suspension cultures in airlift bioreactors: The inhibitory effect at high aeration rates on culture growth. J. Exp. Bot. 37: 1911-1920
Hohe A (1999) Zur somatischen Embryogenese und Vermahrung von Cyclamen persicum Mill. In Bioreaktoren. Dissertation. Vom Fachbereich Gartenbau der Univesitüat Hannover zur Erlangung des akademischen Grades eines
Hohe A, Winkelmann T, Schwenkel HG (1999) CO2 acumulation in bioreactors suspension cultures of Cyclamen persicum Mill. and ist effect on cell growth and regeneration of somatic embryos. Plant Cell Rep. 18: 863-867
Hvoslef-Eide T (2000) Bioreactors for Propagation of Ornamental Plants.EIn: Strømme, E (ed) Advances in Floriculture Research pp. 132-142. Agricultural University of Norway
Ibaraqui Y, Fukakusa M, Kurata K (1995) SOMES2: Imagen-analysis-based somatic embryo sorter. Current Plant Science and Biotech. Letters. 14: 701-706
Jay V, Genestier S, Courduroux JC (1992) Effects of flooding on growth and metabolism of herbaceous plants. En: Kozlowski, T T (Ed) Flooding and plant growth pp. 47-128, Academic Press, New York
Jay V, Genestier S, Courduroux JC (1994) Bioreactor studies of the effect of medium pH on carrot (Daucus carota L.) somatic embryogenesis. Plant Cell, Tissue and Organ Culture. 36: 205-209
Jiménez E, de Feria M, Barbón R, Capote A, Chavéz M (1995) Empleo de biorreactores para la producción de embriones somáticos de café (Coffea arabica cv. Catimor.). En: Avances en biotecnología moderna, libro de reportes cortos, vol.13. p. II.1 – II.2
Joersbo M, Andersen JM, Okkels FT, Rajagopal R (1989) Isoperoxidase as markers of somatic embryogenesis in carrot cell suspension cultures. Physiol Plant 76: 10-16
Johansson L y Erikson T (1984) Effects of carbon dioxide in anther cultures. Physiol. Plant. 60: 26-30
Kawara R, Komamine A (1995) Molecular basis of somatic embryogenesis. En: YPS Bajaj. (Ed.) Biotechnology in Agriculture and Forestry vol 30: 30-40 Springer-Verlag:
Kessell RHJ, Carr AH (1972) The effect of dissolved oxygen concentration on growth and differentiation of carrot (Daucus carota) tissue. J. Exp. Botany 23: 996-1007
Kioyosue T, Satoh S, Kamada H, Harada H (1991) Purification and immunohistochemical detection as embryogenic cell protein in carrot. Plant Physiol 95: 1070-1074
Kitaya Y, Shibuya T, Kozai T, Kubota C (1998) Effects of light intensity and air velocity on air temperature, water vapor presure, and CO2 concentration inside a plant canopy under an artificial lhigting condition. Life Support & Biosphere Science. 5: 199-203
Knox JP (1997) The use of antibodie to study the archietecture and developmental regulation of plant cell walls.Int. Rev. Cytol 171: 79-120
Komalavilas P, Zhu JK, Nothnagel EA (1991) Arabinogalactan proteins from the suspension culture medium and plasma membrane of rose cells. J. Biol Chem 266: 15956-15965
Komamine A (1998) Expresión génica en la embriogénesis somática. En: III Encuentro Latinoaméricano de Biotecnología Vegetal, REDBIO 98. La Habana (1-5 Junio): 516 p.
Kozai T, Kitoya Y, Kubota C, Kobayashi R, Watanabe S (1996) Optimization of photoautotrophic micropropagation conditions for sweetpotato (Ipomea batatas (L.) Lam.) plantets. Acta Hort. 440: 566-569
Kozai T, y Jeong B (1997 Environmental control for the large-sacle production of plants through in vitro techniques. Plant Cell, Tissue and Organ Culture. 51: 49-56
Kreuger M, van Holst GJ (1993) Arabinigalactan proteins are essential in somatic embryogenesis of Daucus carota L. Planta 189: 243-248
Kreuger M, van Holst GJ (1995) Arabinogalactan proteins epitopes in somatic embryogenesis of Daucus carota L. Planta 197: 135-141
Kreuger M, van Holst GJ (1996) Arabinigalactan proteins and plant differentiation. Plant Mol Biol 30: 1077-1086
Kvaalen H, von Arnold S (1991) Effects of various partial pressures of oxygen and carbon dioxide on different satges of somatic embryogenesis in Picea abies. Plant Cell an Organ Culture 27: 49-57
León O (1998) Marcadores moleculares: principales tipos, bases moleculares, ventajas y limitaciones. En: Uso de los marcadores moleculares en la genética y la selección de plantas. Cornide MT (Editora) CNIC.p: 1-18
Litz RE, Schaffer B (1987) Polyamines in adventicious and somatic embryogenesis in mango (Mangifera indica L.). J. Plant Physiol. 128: 251-258
Lukse E, Dinkova TD, Ramos Leal M (1997) Aspectos bioquímicos moleculares de la embriogénesis somática en plantas. Ciencias biológicas 27 (1-2-3): 13-17
Luttman R, Florek P, Preil W (1994) Silicone-tubing aerated bioreactor for somatic embryo production. Plant Cell, Tissue and Organ Culture 39: 157-170
Ma JH, Yao JL, Cohen D, Morris B (1998) Ethylene inhibitors enhance in vitro root formation fromm aple cultures. Plant Cell Reports 17: 211-214
McCabe P, Valentine T, Scott L, Pennell R (1997) Soluble signals from cells identified at the cell wall establish a developmental pathway in carrot. The Plant Cell 9: 2225-2241
Meijer EA, De Vries SC, Mordhorst AP (1999) Co-culture with Daucus carota somatic embryos reveals high 2,4-D uptake and release rates of Arabidopsis thaliana cultured cells. Plant Cell Reports 18: 656-663
Merkle SA, Parrot WA, Flinn BS (1995) Morphogenic aspect of somatic embryogenesis. En. Thorpe, T A (ed), In Vitro embryogenesis in plants: pp. 155 -230 Kluwer Academic Publisher, Dordrecht
Miyoshi K y Sato T (1997) Removal of the pericarp and testa of seeds of japonica and indica rice (Oryza sativa) at various oxygen concentrations has opposite effects on germination. Physiologia Plantarum 99: 1-6
Montague MJ, Koppenbrink JW, Jaworsky EG (1999) Poliamine metabolism in embryogenic cells of Daucus carota I. Changes in intracellular contents and rates of synthesis. Plant Physiol. 62: 430-433
Murphy R, Sites JW, Buth DG, hauflear CH (1990) Proteins I: Isoenzymes electroforesis. En:Hilles DM and Mortiz C. (eds) Molecular systematics pp. 56-87 Sinauer Associats, Suderland
Nguyen QT, Kosai T (1998) Environmental effects on the growth of plantlets in micropropagation. Environ. Control. Biol. 36(2): 59-75
Nguyen QT y Kosai T (1998) Environmental effects on the growth of plantlets in micropropagation. Environ. Control. Biol. 36(2): 59-75
Niu G, Kozai T, Kitaya Y (1996) Simulation of the time courses of CO2 concentration in the culture vessel and net photosynthetic rate of Cymbidium plantlets. Transactions of the ASAE. 39 (4): 1567-1573
Niu G y Kosai T (1997) Simulation of the growth of potato plantlets cultured photoautotrophically in vitro. Transactions of the ASAE. 40(1): 255-260
Nohtnagel EA (1997) Proteoglycans and related components in plant cell. Int. Rev. Cytol. 174: 195-291
Noualle CH, Petiart, V (1988) Semecens articielles: reves et reálités. Serpe MD, Nothnagel EA (1995) Fractionation and the structural
Biofutur 67: 33-38 characterization of arabinogalactan proteins on the plasma
membrane of rose cells. Plant Physiol 109: 1007-1016
Okamoto A, Kishine S, Hirosawa T, Nakazono A (1996) Effect of oxygen enrriched aireation on regeneration of rice (Oryza sativa L.) cell culture. Plant Cell Report 15: 731-736
Pennel RI, Jannice L, Scofield GN, Booij H, de Vries SC, Roberts K (1992) Identification of a transitional cell state in the developental pathway of carrot somatic embryogenesis. The Journal of Cell Biology 119: 1371-1380
Poulsen GB, Frugis G, Albrechten M, Marroti D (1996) Synthesis of extracellular proteins in embryogenic and non-embryogenic cell cultures of alfalfa. Plant Cell Tiss Org Cult 44: 257-260
Preil W y Beck A (1991) Somatic embryogenesis in bioreactor culture. Acta Horticulturae 289: 179-192
Preil W, Bertram A, Henning J, Schneidereit M (1996) Comparison of growth and nutrient uptake of embriogenic Cyclamen persicum cultivated in Erlenmeyer flask and bioreactor with rotating and vibrating stirrer. COST 822 Working Group 2, Abstracts of the Third Meeting of the working Group
Preil W, Krause I, Schneidereit M (1998) Effects of CO2 and O2 on growth of embryogenic Cyclamen persicum bioreactor cultures. COST 822 Working Group 2, Abstracts of the Fifth Meeting of the working Group
Preil W (1991) Aplication of bioreactor in plant propagation. En: Debergh, P C, Zimmerman, R H (eds.). Micropropagation, technology and aplication. pp.425-445 Klumer Academic Publishers Dordrecht, Boston, London
Preil W, Florek P, Wix U, Beck A (1988) Towards mass propagation by use of bioreactors. Acta Hort. 226: 99-106
Reinert J (1958) Untersuchungen uber die morphogenese and gewebwkulturen. Ver. Dtsc. Bot. Ges. 71: 15-51
Rodríguez M, Lukse E, Maribona RH, Ramos Leal M (1996) Análisis inmunoquímico de la embriogénesis somática de la caña de azúcar. X Seminario Científico. INCA: 100
Rodríguez M, Niubó E, Maribona RH (1999) Análisis inmunoquímico de la embriogénesis somática de suspensiones celulares de caña de azúcar. Revista CENIC. Ciencias biológicas 30(2): 25-32
Roustan JP, Latché A, Fallot J (1989) Stimulation of Daucus carota L. somatic embryogenesis by inhibitors of ethylene Synthesis: cobalt and nickel. Plant Cell Report 8: 182-185
Roustan JP, Latché A, Fallot J (1990) Inhibition of ethylene production and stimulation of carrot somatic embryogenesis by salicilic acid. Biol. Plant 32: 273-275
Saare-Surmisnski K, Preil W, Knox P, Lieberei R (2000) Arabinogalactan proteins in embryogenic and not-embryogenic callus cultures of Euforbia pulcherrima. Physiologia Plantarum 108: 180-187
Sakano K, Kiyota S, Yazaki Y (1997) Acidification and alkalinization of culture medium by Catharanthus roseus cells is anoxic production of lactate a cause of citoplasmic acidification? Plant Cell Physiol. 38: 1053-1059
Samaj J, Baluška F, Bobàk M, Volkmann D (1999) Extracellular matrix surface network of embriogenic units of friable maize callus contains arabinogalactan-proteins recognized by monoclonal antibody JIM4. Plant cell Reports 18: 369-374
Sánchez MD (1999) Curso de especialización en recursos fitogenéticos. Sección 2: caracterización de germoplasma vegetal. Departamento de biología Vegetal. E.T.S. de Ingenieros Agrónomos, Madrid
Shigeta J, Sato K, Mii M (1996) Effects of initial cell density, pH and dissolved oxygen on bioreactor production of carrot somatic embryos. Plant Science 115: 109-114
Shimazu T, Kurata K (1999) Relationship between production of carrot somatic embryos and dissolved oxygen concentration in liquid culture. Plant Cell, Tissue and Organ Culture 57: 29-38
Slay RM, Grimes HD, Hodges TK (1989) Plasme membrane proteins associated with undeferentieted and embryogenic Daucus carota tissue. Protoplasma. 139: 149-151
Stacey NJ, Roberts K, Knox JP (1990) Patterns of expression of the JIM4 arabinogalactan-protein epitope in cell cultures and during somatic embryogenesis in Daucus carota L. Planta 180: 285-292
Stejskal J, Griga M (1985) Comparative analysis of isoenzymes and proteins in somatic and zygotic embryos of soybean (Glycine max Merr. ) J. Plant Physiol 146: 497-502
Sterk P, Booij H, Gerard A, Schellekens A, van Kammen A, de Vries SC (1991) Cell specific expresion of the carrot EP2 lipid transfer protein gene. The plant Cell 3: 907-921
Sung Z, Okimoto R (1981) Embryogenic proteins in somatic embryos of carrot. Proc. Natl. Acad. Sci. USA 78 (6): 3683-3687
Sung ZR, Lazar GB, Dudits D (1981) Cyclohexamide resistance in carrot culture: a differentiated function. Plant Physiol 68: 261-264
Szabados L, Mroginski LA y WM Roca (1993) Suspensiones celulares: descripción, manipulación y aplicaciones. En: Roca, W M y L A Mroginski (Eds). Cultivo de Tejidos en la Agricultura. pp. 173-210 CIAP. CALI
Thompson MR, Thorpe TA (1989) Biochemical aspects of crop improvement. (Khama Eds) CRC Press Boston
Thorpe TA (1988) Organogenesis in vitro. Structural, physiological and biochemical aspects. En: IK Vasil (eds) International reviews of Cytology, Suplement 11A, perspectives in Plant cell and Tissue Culture pp. 71-112, Academic Press, New York
Toonen M, Schmidt EDL, de Vries SC (1996) Cell tracking as a tool to study initial processes in somatic embryo development. Plant Tissue and Biotechnology 2: 3-10
Toonnen MAJ, Schmidt EDL, van Kammen A, de Vries SC (1997) Promotive andd inhibitory effects of diverse arabinogalactano proteins on Daucus carota L. somatic embryogenesis. Planta 203: 188-195
van Engelen FA, Sterk P, Booij H, Cordewener J, Kook W, van Kammen A, de Vries SC (1991) Heterogeneity and cell type-specific localization of a cell wall glycoprotein from carrot suspensions cells. Plant Physiol 96: 705-712
van Engelen FA y SC de Vries (1992) Extracellular proteins in plant embryogenesis. Trends Genet. 8: 66-90
Veisseire P, Cailloux F, Coudret A (1994) Effect of conditioned media on the somatic embryogenesis of Hevea brasiliensis. Plant Physiol Biochem 32: 571-576
Wurtele ES, Wang H, Durgerian S, Nikolau BJ, Ulrich TH (1993) Characterization of gene that is expressed early in somatic embryogenesis of Daucus carota. Plant Physiol. 102: 303-312
Yadav J S, Rajam MV (1998) Temporal regulation of somatic embryogenesis by adjusting cellular content in eggplant. Plant Physiol 116: 617-625
Yariv J, Rapport MM, Graf L (1962) The interaction of glycosides and saccharides with antibody of the corresponding phenylazo glycosides. Biochem. J. 85: 383-388
Zhu, JK, Bressan RA, Hasegawa PM (1993) Loss of arabinogalactan-proteins from the plasma membrane of NaCl-adapted tobacco cells. Planta 18: 221-226
Zimmerman JL (1993) Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5: 1411-1423
Ziv M (1995) The control of bioreactor environment for plant propagation in liquid culture. Acta Horticulturae. 393: 25-38
Zobayed SMA, Afreen- Zobayed, Kubota C, Kozai T (1999) Large-scale photoautotrophic micropropagation of Eucaliptus plantlets under forced ventilation. Abstr. The congress on in vitro Biology
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