Virus de la mancha anular de la papaya (PRSV-p): Biología, epifitiología y diversidad genética como base para el manejo mediante técnicas biotecnológicas
Resumen
El Virus de la mancha anular de la papaya constituye en muchos países tropicales y subtropicales el mayor obstáculo en la producción de papaya y es responsable de pérdidas considerables en las cosechas. El modo de transmisión de este virus impide el éxito de los tratamientos con insecticidas. Además, la presencia de áfidos vectores durante todo el año en las plantaciones constituye una importante vía para la distribución de la enfermedad. Una vez infectadas las plantaciones no existe tratamiento eficaz para su control. En varios países se han desarrollado estrategias mediante la ingeniería genética, donde se ha logrado obtener buenos resultados, lo que implica el estudio biológico y molecular de los posibles aislados del PRSV que se pueden presentar en una región o país. En Cuba, se han realizado investigaciones encaminadas al desarrollo de estrategias para el manejo de esta enfermedad viral. Este trabajo tuvo como objetivo relacionar los principales aspectos biológicos y epifitiológicos del PRSV, con resultados de Cuba y el mundo, como herramienta para el manejo de esta enfermedad mediante el uso de la biotecnología vegetal.
Palabras clave: áfidos, proteína de la cápsida, PRSV
Referencias
Acuña, J, Zayas F (1946) El mosaico y otras enfermedades de la fruta bomba (Carica papaya L.). Circular 85, Estación Experimental Agronómica de Santiago de las Vegas. La Habana
Adams, MJ, Antoniw JF, Fauquet CM (2004) Molecular criteria for genus and species discrimination whithin the family Potyviridae. Archives of Virology 150: 459-479
Agrios, GN (2005) Plant pathology. 5ta ed. Elsevier, San Diego, California
Arocha, Y, Jones P (2007) Potyvirus identified in papaya in the 2007 survey in Cuba. [en línea] en: http://www.ncbi.nlm.nih.gov [consulta: 8 diciembre 2008]
Atreya, PL, Atreya CD, Pirone TP (1991) Amino acid subtitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proceedings of the National Academic of Sciences USA 88: 7887-7891
Atreya, PL, Lopez-Moya JJ, Chu M, Atreya ChD, Pirone TP (1995) Mutational analysis of the coat protein N-terminal amino acids involved in potyvirus transmission by aphids. Journal of General Virology 76: 265-270
Badillo, VM (2000) Carica L. vs Vasconcellea St. Hil. (Caricaceae) con la rehabilitación de este último. Ernstia 10: 74-99
Barreto, PD, dos Santos AA, Dantas JLL (2002) Genótipos de mamão sob infecção natural pelo vírus da mancha-anelar. Revista Ciência Agronômica 33: 43-47
Bateson, MF, Henderson J, Chaleeprom W, Gibbs AJ, Dale JL (1994) Papaya ringspot potyvirus: isolate variability and the origin of PRSV type P (Australia). Journal of General Virology 75: 3547-3553
Bateson, MF, Lines RE, Revill P, Chaleeprom W, Ha CV, Gibbs AJ, Dale JL (2002) On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus. Journal of General Virology 83: 2575-2585
Bayot, RG, Villegas VN, Magdalita PM, Jovel-Lana MD, Espino TM, Exconde SB (1990) Seed transmissibility of Papaya ringspot virus. Philippine Journal of Crop Science 15: 107-111
Berger, PH, Wyatt SD, Shiel PJ, Silbernagel MJ, Druffel K, Mink GI (1997) Phylogenetic analysis of the Potyviridae with emphasis on legume-infecting potyviruses. Archives of Virology 142: 1979-1999
Blackman, RL, Eastop V (2007) Taxonomic Issues. En: Van Emden, HF, Harrington R (Eds) Aphids as crop pests, pp. 1-30. CAB International, Wallingford
Bousalem, M, Douzery EJP, Fargette D (2000) High genetic diversity, distant phylogenetic relationships and intraspecies recombination events among natural populations of Yam mosaic virus: a contribution to understanding potyvirus evolution. Journal of General Virology 81: 243-255
Cabrera, D, Portal O, Cruz M, Hernández R (2009a) Diagnostic and biological characterization of a Papaya rinspot virus isolate (PRSV-p) from Cienfuegos, Cuba. Phytopathology 99: S189
Cabrera, D, Sosa R, Portal O, Alburquerque Y, González JE, Hernández R (2009b) Alterations induced by Papaya ringspot potyvirus on chlorophyll content in papaya (Carica papaya L.) leaves. Fitosanidad 13: 125-126
Cabrera, D, Cruz M, Portal O (2010) Efecto de la temperatura en la virulencia del Virus de la mancha anular de la papaya (PRSV-p). Fitosanidad 14: 123-125
Chiang, CH, Lee CY, Wang CH, Jan FJ, Lin SS, Chen TC, Raja JAJ, Yeh SD (2007) Genetic analysis of an attenuated Papaya ringspot virus strain applied for cross-protection. European Journal of Plant Pathology 118: 333-348
Chin, M, Ahmad MH (2007) Momordica charantia is a weed host reservoir for Papaya ringspot virus type p in Jamaica. Plant Disease 91: 1518
Conover, RA (1964) Distortion ringspot, a severe virus disease of papaya in Florida. Proceedings of the Florida State Horticultural Society 77: 440-444
Dimmock, NJ, Easton AJ, Leppard KN (2007) Introduction to modern virology. 6ta ed. Blackwell Publishing Ltd. Maldem, MA
Drake, JW, Charlesworth B, Charlesworth D, Crow JF (1998) Rates of spontaneous mutation. Genetic 148: 1667-1686
Drake, JW y Holland JJ (1999) Mutation rates among RNA viruses. Proceedings of the National Academy of Sciences USA 96: 13910-13913
FAO (2009) FAOSTAT, FAO Statistics Division. Crops production quantity. [en línea] en: http:// faostat.fao.org/site/567/default.aspx [consulta: 8 de septiembre de 2009]
Fariñas, ME, López E (1986) Enfermedades virales en diferentes localidades productoras de frutabomba en Cuba. Simposio de Citricultura Tropical. La Habana, Cuba
Fauquet, CM, Mayo MA, Maniloff J, Desselbelguer U, Ball LA (2005) Virus Taxonomy: The Eighth Report of the International Committee on Taxonomy of Viruses. Elsevier. Amsterdam
Fermín, G, Inglessis V, Garboza C, Rangel S, Dagert M (2004) Engineered resistance against Papaya ringspot virus in Venezuelan transgenic papayas. Plant Disease 88: 516-522
Fernández-Rodríguez, T, Rubio L, Carballo O, Marys E (2008) Genetic variation of Papaya ringspot virus in Venezuela. Archives of Virology 153: 343-349
Fitch, MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1992) Virus resistance papaya plants
derived from tissues bombarded with the coat protein gene of Papaya ringspot virus. Bio-Technology 10: 1466-1472
García-Arenal, F, Fraile A, Malpica JM (2001) Variability and genetic structure of plant virus populations. Annual Review of Phytopathology 39: 157-86
Gonsalves, D, Ishii M (1980) Purification and serology of Papaya ringspot virus. Phytopathology 70: 1028-1032
Gonsalves, D (1993) Papaya ringspot virus (P-strain). [en línea] en: http://www.extento.hawaii.edu/ kbase/ Crop/Type/papring.htm [consulta: 8 diciembre 2009]
Gonsalves, D (1998) Control of Papaya ringspot virus in papaya: a case study. Annual Review of Phytopathology 36: 415-437
Gonsalves, D (2002) Coat protein transgenic papaya ‘acquired’ immunity for controlling Papaya ringspot virus. Current Topics in Microbiology and Immunology 266: 73-83
Gonsalves, D, Ferreira S, Manshardt R, Fitch M, Slightom J (1998) Transgenic virus resistant papaya: New hope for control of Papaya ringspot virus in Hawaii. APSnet Feature, American Pythopathological Society. [en línea] en: http://www.apsnet.org/education/ feature/papaya [consulta: 5 de marzo de 2009]
González, G, Mejías Y, Rodríguez D (1988) Virus de la mancha anular de la fruta bomba (Papaya ringspot virus) en Cuba. Ciencia y Técnica en la Agricultura, Serie Protección de Plantas 11: 17-32
González, L, Rodríguez D (2008) Dinámica poblacional de vectores del Virus de la mancha anular de la papaya intercalada con naranja valencia y diseminación de la enfermedad. Centro Agrícola 35: 55-60
Hari, V, Siegel A, Rozek D, Timberlake WE (1979) The RNA of Tobacco etch virus contains poly(A). Virology 92: 568-571
Hernández, R (1994) Estudio sobre el Virus de la mancha anular de la fruta bomba (Carica papaya L.). Señalización de vectores y control e integración con otras medidas fitosanitarias. Tesis en opción al grado científico de Doctor en Ciencias Agrícolas. IBP, Universidad Central ‘Marta Abreu’ de Las Villas, Santa Clara
Inoue-Nagata, AK, Franco CD, Martin DP, Martin DP, Rezende JA, Ferreira GB, Dutra LS, Nagata T (2007) Genome analysis of a severe and a mild isolate of Papaya ringspot virus-type W found in Brazil. Virus Genes 35: 119-127
Jain, RK, Pappu HR, Pappu SS, Varma A, Ram RD (1998) Molecular characterization of Papaya ringspot potyvirus isolates from India. Annals of Applied Biology 132: 413-425
Jain, RK, Sharma J, Sivakumar AS, Sharma PK, Byadgi AS, Verma AK, Varma A (2004) Variability in the coat protein gene of Papaya ringspot virus isolates from multiple locations in India. Archives of Virology 149: 2435-2442
Jensen, DD (1949) Papaya virus diseases with special reference to papaya ringspot. Phytopathology 39: 191-211
Jiang, L, Maoka T, Komori S, Fukamachi H, Kato H, Ogawa K (2005) An efficient method for sonification assisted Agrobacterium-mediated transformation of coat protein (CP) coding genes into papaya (Carica papaya L.). Plant Cell Reports 24: 426-432
Lima, RCA, Souza JMT, Pio-Ribeiro G, Lima JAA (2002) Sequences of the coat protein gene from Brazilian isolates of Papaya ringspot virus. Fitopatologia Brasileira 27: 174-180
López-Moya, JJ, López-Abella D (1995) Transmisión de virus de plantas por insectos vectores. En: Llacer, G, López MM, Trapero A, Bello A (Eds) Patología Vegetal, pp. 275-300. Sociedad Española de Fitopatología, Madrid
López-Moya, JJ, Wang RY, Pirone TP (1999) Context of the coat protein DAG motif affects potyvirus transmissibility by aphids. Journal of General Virology 80: 3281-3288
MINAGRI (2008) Instructivo técnico del cultivo de la fruta bomba. INIVIT. Villa Clara
Mishra, M, Chandra R, Saxena S (2007) Papaya. En: Kole, C (Ed) Genome mapping and molecular breeding in plants, pp. 343-351. Springer-Verlag, Heidelberg
Morales, A, Medina D, Yaguachi B (2004) Diversidad genética, filogenética y distribución geográfica del género Vasconcellea en Sur de Ecuador. Lyonia 7: 15-27
Ng, JCK, Perry KL (2004) Transmission of plant viruses by aphid vectors. Molecular Plant Pathology 5: 505-511
Noa-Carrazana, JC, González-de-León D, Ruiz-Castro BS, Piñero D, Silva-Rosales L (2006) Distribution of Papaya ringspot virus and Papaya mosaic virus in papaya plants (Carica papaya) in Mexico. Plant Disease 90: 1004-1011
Ohshima, K, Yamaguchi Y, Hirota R, Hamamoto T, Tomimura K, Tan Z, Sano T, Azuhata F, Walsh JA,
Fletcher J, Chen J, Gera A, Gibbs A (2002) Molecular evolution of Turnip mosaic virus: evidence of host adaptation, genetic recombination and geographical spread. Journal of General Virology 83: 1511-1521
Pérez, LF, González G (2007) Enfermedades del Papayo: descripción, epidemiología y manejo. Editorial Científico-Técnica, La Habana
Persley, D (2005) Papaya Ringspot Disease. Department of Primary Industries and Fisheries. Queensland Government. [en línea] en: http:// www2.dpi.qld.gov.au/horticulture/5333.html [consulta: 8 diciembre de 2009]
Pirone, TP, Perry KL (2002) Aphids-nonpersistent transmission. Advances in Botanical Research 36: 1-19
Portal, O, Cabrera D, Sánchez A, Darías AL, Gonzáles JE, Gómez R (2006) Molecular characterization of two Cuban isolates of the Papaya ringspot virus by means of coat protein analysis. Communication of Agricultural Applied Biological Sciences 71: 1203-1205
Purcifull, DE, Edwardson JR, Hiebert E, Gonsalves D (1984) Papaya ringspot virus. CMI/AAB Descriptions of Plant Viruses No. 292
Quemada, H, Hostis BL, Gonsalves D, Reardon IM, Heinrikson R, Hiebert EL, Sieu LC, Slightom JL (1990) The nucleotide sequence of the 3' terminal regions of Papaya ringspot virus strains W and P. Journal of General Virology 71: 203-210
Raccah, B, Huet H, Blanc S (2001) Potyviruses. En: Harris, KF, Smith OP y Duffus JE (Eds) Virus-insect-plant interactions, pp.181-206. Academic Press, New Yo r k
Riechmann, JL, Laín S, Garcia JA (1989) The genome-linked protein and 5' end RNA sequence of Plum pox potyvirus. Journal of General Virology 70: 2785-2789
Riechmann, JL, Laín S, Garcia JA (1992) Highlights and prospects of potyvirus molecular biology Journal of General Virology 73: 1-16
Rieger, M (2006) Introduction to Fruit Crops. The Haworth Press, New York
Roig, JT (1965) Diccionario Botánico de Nombres Vulgares Cubanos. 3ra ed, Editora del Consejo Nacional de Universidades, La Habana
Shukla, DD, Strike PM, Tracy SL, Gough KH, Ward CW (1988) N and C termini of the coat protein of potyviruses are surface-located: The N terminus contains the major virus-specific epitopes. Journal of General Virology 69: 1497-1508
Shukla, DD, Ward CW (1988) Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69: 2703-2710
Shukla, DD, Ward CW (1989a) Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106: 171-200
Shukla, DD, Ward CW (1989b) Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Advances in Virus Research 36: 273-314
Shukla, DD, Ward CW, Brunt AA, Berger PH (1998) Potyviridae family. AABDPV Descriptions of Plant Viruses No. 366
Silva-Rosales, L, Becerra-Leor N, Ruíz-Castro S, Téliz-Ortiz D, Noa-Carrazana JC (2000) Coat protein sequence comparisons of three Mexican isolates of Papaya ringspot virus with other geographical isolates reveal a close relationship to American and Australian isolates. Archives of Virology 145: 835-843
Souza, MT, Nickel O, Gonsalves D (2005) Development of virus resistanttransgenic papayas expressing the coat protein gene from a Brazilian isolate of Papaya ringspot virus. Fitopatologia Brasileira 30: 357-365
Spetz, C, Taboada AM, Darwich S, Ramsell J, Salazar L F, Valkonen JPT (2003) Molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in Peru. Journal of General Virology 84: 2565-2578
Tecson-Mendoza, EM, Laurena AC, Botella R (2008) Recent advances in the development of transgenic papaya technology. Biotechnology Annual Review 14: 423-462
Tennant, P, Ahmad MH, Gonsalves D (2005) Field resistance of coat protein transgenic papaya to Papaya ringspot virus in Jamaica. Plant Disease 80: 841-847
Tennant, PF, Gonsalves C, Ling KS, Fitch M, Manshardt R, Slightom JL, Gonsalves D (1994) Differential protection against Papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya. Phytopathology 84: 1359-1366
Thomas, JE, Dodman RL (1993) The first record of Papaya ringspot virus type P in Australia. Australasian Plant Pathology 22: 2-7
Tripathi, S, Suzuki JY, Ferreira SA, Gonsalves D (2008) Papaya ringspot virus-P: characteristics, pathogenicity, sequence variability and control. Molecular Plant Pathology 9: 269-280
Urcuqui-Inchima, S, Haenni AL, Bernardi F (2001) Potyvirus proteins: a wealth of functions. Virus Research 74: 157-175
Vegas, A, Gonzalez A, Trujillo G, Pino I (1998) Dificultad en el diagnóstico serológico de cepas atenuadas del Virus de la mancha anillada distorsionante de la lechosa (PRSV). Fitopatología Venezolana 11: 40-44
Yeh, SD, Gonsalves D (1985) Translation of Papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical inclusion protein and amorphous inclusion protein. Virology 143: 260-271
Yeh, SD, Bau HJ, Kung YJ y Yu TA (2007) Papaya. En: Pua, EC, Davey MR (Eds) Biotechnology in Agriculture and Forestry, pp. 73-96. Springer-Verlag, Heidelberg
Yeh, SD, Jan FJ, Chiang CH, Doong TJ, Chen MJ, Chung PH, Bau HJ (1992) Complete nucleotide sequence and genetic organization of Papaya ringspot virus RNA. Journal of General Virology 73: 2531-2541
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