Determinación de la estabilidad genética de plantas propagadas in vitro de Vasconcellea x helbornii Badillo (Badillo) con el uso de marcadores ISSR

Mónica Jadán, Shirley Brito, Claudia Segovia, Rafael Gómez-Kosky, Idalmis Bermúdez-Caraballoso, Karina Proaño

Resumen


Los papayuelos de montaña, entre ellos el babaco [Vasconcellea x helbornii Badillo (Badillo)], son   originarios del Ecuador y poseen un alto valor nutricional por lo que tiene gran demanda a nivel nacional e internacional. El presente trabajo tuvo como objetivo determinar a través de marcadores ISSR la estabilidad genética de plantas de babaco propagadas in vitro. Se utilizaron hojas jóvenes (uno y dos) de 10 plantas madre (estacas) y de 20 plantas propagadas in vitro.  Para el análisis se emplearon ocho cebadores. Un total de 55 bandas fueron observadas de las 30 plantas analizadas. Se observó un 100% de loci monomórficos entre las plantas madre provenientes de estacas y las plantas propagadas in vitro. Este análisis indicó que no existieron diferencias a nivel molecular entre las plantas analizadas, para las combinaciones de cebadores empleadas y que los marcadores ISSR pueden ser usados para este propósito.

 

Palabras clave: babaco, marcadores moleculares, organogénesis, variación somaclonal


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Referencias


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