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Bacillus megaterium: bacteria endofítica de callos de Ilex paraguariensis con actividad de promoción del crecimiento
Resumen
Los microorganismos endófitos viven dentro de plantas sanas y su aislamiento e identificación pueden favorecer las aplicaciones biotecnológicas. Los microorganismos endofíticos fueron encontrados en callos de Ilex paraguariensis cultivados in vitro durante cinco meses. Se usó la secuenciación de la región 16S rRNA para identificar dos aislados como Bacillus megaterium. La presencia de células bacterianas en los espacios intercelulares de los cultivos de callos se detectó mediante análisis ultraestructurales. Los aislamientos también se evaluaron para la producción de ácido indol acético (AIA) y su potencial en la promoción del crecimiento de las plántulas de Phaseolus vulgaris L. La síntesis de ácido AIA en presencia y ausencia de L-triptófano se detectó por análisis colorimétrico para ambos aislamientos. En presencia de extractos de las cepas IPC06 e IPC07, las plántulas de P. vulgaris crecieron más rápido en comparación con las plantas control sin el extracto en pruebas in vitro.
Palabras clave: yerba mate, 16S rRNA, secuenciación, cultivo de tejidos
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