Aspectos básicos de la propagación in vitro del género Pinus por organogénesis

Maité Chávez, Manuel de Feria

Resumen


La regeneración de plantas in vitro del género Pinus por embriogénesis somática ha sido muy estudiada. Sin embargo, el establecimiento in vitro de plantas a partir brotes apicales y su multiplicación han sido alternativas menos analizadas y en consecuencia se ha limitado la obtención de plantas regeneradas por organogénesis capaces de logar con éxito la supervivencia en la fase de aclimatización. El presente trabajo se realizó con el objetivo de presentar una revisión de la literatura científica sobre la propagación in vitro por organogénesis del género Pinus, incluyendo un análisis de los principales factores que de alguna forma podrían afectar el éxito de la regeneración de plantas por este método, con énfasis en la formación de raíces tanto in vitro como ex vitro.

 

Palabras clave: brotes apicales, enraizamiento ex vitro, enraizamiento in vitro, pino, regeneración de plantas.


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Referencias


Agrawal, V, Prakash S, Gupta SC (2002) Effective protocol for in vitro shoot production through nodal explants of Simmondsia chinensis. Biol. Plant 45: 449-453

Aitken-Christie, J, Singh A, Davies H (1986) Multiplication of meristematic tissue culture system for radiata pine. Biotechnology in Agriculture and Forestry 1: 413-432

Alkhateeb, AA (2001) Influence of different carbon sources and concentrations on in vitro root formation of date palm (Phoenix dactylifera L.) cv Khanezi. Zagazig J. Agric. Res. 28: 597-608

Alkhateeb, AA (2008) Comparison effects of sucrose and date palm syrup on somatic embryogenesis of date palm (Phoenix dactylifera L.). American Journal of Biotechnology and Biochemistry 4: 19-23

Alonso, P, Moncaleán P, Fernández B, Rodríguez A, Centeno ML, Ordás RJ (2006) An improved micropropagation protocol for stone pine (Pinus pinea L.). Ann. For. Sci. 63: 879-885

Amerson, HV, McKeand SE, Mott RL (1981) Tissue culture and greenhouse practices for the production of loblolly pine plantlets. Proceedings of the 16th Southern Forest Tree Improvement Conference. (Conference Proceedings) pp. 168-175

Arigita, L, Gonzalez A, Tamés RS (2002) Influence of CO2 and sucrose on photosynthesis and transpiration of Actinidia deliciosa explants cultured in vitro. Physiologia Plantarum 115: 166-173

Ben-Jaacov, J, Ackerman A, Tal E, Jacobs G (1991) Vegetative propagation of Alberta magna by tissue culture and grafting. HortScience 26: 74

Bonga, JM, Klimaszewska KK, von Aderkas P (2010) Recalcitrance in clonal propagation, in particular of conifers. Plant Cell, Tissue and Organ Culture 100: 241-254

Bonga, JM, Von Aderkas P (1992) In vitro culture of trees. Kluwer Academic Publishers, Dordrecht

Calamar, A, De Klerk GJ (2002) Effect of sucrose on adventitious root regeneration in apple. Plant Cell, Tissue and Organ Culture 70: 207-212

Cantillo, R, Igarza J, Ochoa AM (2011) Propagación in vitro de plantas de Pinus cubensis Griseb. Biotecnología Vegetal 11 (1): 3-13

Cárdenas, LA, Villegas MA (2002) Potencial osmótico del medio de cultivo con diferentes componentes para la propagación in vitro. Rev. Fitotec. Mex. 25: 213-217

Chávez, M, de Feria, M, Barbón, R, La O, M, Pérez, M, Jiménez-Terry, F, Quiala, E, Agramonte, D (2010) Características morfológicas de plantas in vitro de Pinus caribaea var. caribaea influenciadas por el empleo de la sacarosa en la fase de multiplicación. Biotecnología Vegetal 10 (1): 31-40

Coke, J (1996) Basal nutrient medium for in vitro cultures of loblolly pines. USA Patent 5,534,433 y 5,534,434

De Diego, N, Montalbán IA, Fernández E, Montcaleán P (2008) In vitro regeneration of Pinus pinaster adult trees. Can J For Res. 38: 2607-2615

de Feria, M, Chávez, M, Barbón, R, La O, M, Pérez, M, Jiménez-Terry, F, Quiala, E, Agramonte, D (2008) Establecimiento in vitro de brotes apicales de Pinus caribaea var. caribaea. Biotecnología Vegetal 8 (1): 15-20

de Feria, M, Chávez, M, Barbón, R, La O, M, Pérez, M, Jiménez-Terry, F, Quiala, E, Agramonte, D (2009) Multiplicación in vitro de plantas de Pinus caribaea var. caribaea. Biotecnología Vegetal 9 (4): 217-224

Ewald, D (2007) Micropropagation of Larix species via organogenesis. En: Jain, SM y Häggman H (Eds) Protocols for Micropropagation of Woody Trees and Fruits, pp. 125-136. Springer, Dordrecht

Frampton, J, Amerson, H, Leach G (1998) Tissue culture method affects ex vitro growth and development of loblolly pine. New Forests 16: 125-138

Fuentes, G, Talavera C, Oropeza C, Desjardins Y, Santamaria JM (2005) Exogenous sucrose can decrease in vitro photosynthesis but improve field survival and growth of coconut (Cocos nucifera L.) in vitro plantlets. In Vitro Cellular Development Biology Plant 41: 69-76

Gamborg, O, Murashige T, Thorpe T, Vasil I (1976). Plant Tissue Culture Media. In Vitro 12: 473-478

Gibson, SI (2000) Plant sugar-response pathways. Part of a complex regulatory web. Plant Physiology 124: 1532-1539

Gupta, PK, Durzan DJ (1985) Shoot multiplication from mature trees of Douglas-fir (Pseudotsuga menziesii) and sugar pine (Pine lambertiana). Plant Cell Report 4: 177-179

Haines, R, Copley T, Huth J, Nester M (2000) The relationship between morphological features and the rooting of cuttings of Pinus caribaea var. hondurensis x Pinus tecunumanii. Forestry Research Centre, Queensland, Australia

Kalia, RK, Arya S, Kalia S, Arya I, Sharma S (2000) Plantlet production in Chir Pine through axilary bud proliferation. Peer Review Meeting. [En línea] En: http://dr.satishkant.port5.com/pineaxillary.pdf (Consultado el 12 de diciembre de 2006)

Kalia, RK, Arya S, Kalia S, Arya ID (2007) Plantlet regeneration from fascicular buds of seedling shoot apices of Pinus roxburghii Sarg. Biol Plant 51: 653-659

Klimaszewska, K, Trontin JF, Becwar MR, Devillard C, Park YS, Lelu MA (2007) Recent Progress in Somatic Embryogenesis of Four Pinus spp. Tree and Forestry Science and Biotechnology 1: 11-25

Korban, SS, Sul IW (2007) Micropropagation of coast Redwood (Sequoia sempervirens) En: Jain, SM y Häggman H (Eds) Protocols for Micropropagation of Woody Trees and Fruits, pp. 23-32. Springer, Dordrecht

Kubota, C, Ezawa M, Kozai T, Wilson SB (2002) In situ estimation of carbon balance of in vitro sweet potato and tomato plantlets cultured with varying initial sucrose concentrations in the medium. J. Am. Soc. Hort. Sci. 127: 963-970

Kumar, R, Sharma K y Agrawal V (2005) In vitro clonal propagation of Holarrhena antidysenterica (L.) Wall. through nodal explants from mature trees. In Vitro Cellular and Developmental Biology - Plant 41: 137-144

Lelu, MA, Michele W, Klimaszewska K (2006) Simplified and improved somatic embryogenesis for clonal propagation of Pinus pinaster (Ait.). Plant Cell Report 25: 767-776

Maruyama, E, Hosoi Y, Ishii K (2007) Somatic embryogenesis and plant regeneration in yakutanegoyou, Pinus armandii Franch. var. amamiana (Koidz.) Hatusima, an endemic and endangered species in Japan. In Vitro Cellular and Developmental Biology-Plant 43: 28-34

Mathur, G, von Arnold S, Nadgauda RS (2001) Abstract En: 8th Meeting of The conifer Biotechnology Working Group, Douglass College Centre, Rutgers University, New Brunswick, New Jersey, June 7-11

Murashige, T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15: 473-497

Nandwani, D, Kumaria S, Tandon P (2001) Micropropagation of Pinus kesiya Royle ex Gord (Khasi pine). Gartenbauwissenschaft 66: 68-71

Nehra, NS, Becwar MR, Rottmann WH, Pearson L, Chowdhury K, Chang S, Wilde HD, Kodrzycki RJ, Zhang C, Gause KC, Parks DW, Hinchee MA (2005) Forest biotechnology: innovative methods, emerging opportunities. In Vitro Cellular and Developmental Biology _ Plant 41: 701-717

Niella, F, Rocha P (2004) Biotecnologías aplicadas a los programas de mejoramiento genético de Pinus spp. en la región. Jornadas de mejoramiento genético para productores forestales. Julio 15-16, 2004. FCF-UNaM-INTA-BDP Posadas, Misiones-Argentina

Niemi, K, Vuorinen T, Ernstsen A, Häggman H (2002) Ectomycorrhizal fungi and exogenous auxins affect root and mycorrhiza formation on in vitro Scots pine hypocotyl cuttings. Tree Physiology 22: 1231-1239

Ojeda, MC(1996) Inducción de organogénesis y embriogénesis somática en Pinus cembroides (Zucc) y Pinus halepensis Mill. (Mill). Tesis para optar al grado de Maestro en Ciencias en Producción Agrícola. Universidad Autónoma de Nuevo León. Fac. Agronomía. 58 p.

Oliveira, P, Barriga J, Cavaleiro C, Peixe A, Potes AZ (2003) Sustained in vitro root development obtained in Pinus pinea L. inoculated with ectomycorrhizal fungi. Forestry 76: 579-587

Parasharami, V, Poonawala I, Nadgauda R (2003) Bud break and plantlet regeneration in vitro from mature trees of Pinus roxburghii Sarg. Current Science 84: p 25

Pawlicki, N, Welander M (1995) Influence of carbohydrate source, auxin concentration and time of exposure in adventitious rooting of the apple rootstock Jork 9. Plant Science 106: 167-176

Prehn, D, Serrano C, Mercado A, Stange C, Barrales L, Arce-Johnson P (2003) Regeneration of whole plants from apical meristems of Pinus radiata. Plant Cell, Tissue and Organ Culture 73: 91-94

Premkumar, A, Barcelo A, Pliego F, Quesada MA, Mercado JA (2002) Influences of exogenous sucrose on juvenile avocado during in vitro cultivation and subsequent ex vitro acclimatization. Trees 16: 569-575

Pullman, GS, Johnson S, Van Tassel S, Zhang Y (2005) Somatic embryogenesis in loblolly pine (Pinus taeda L.) and Douglas fir (Pseudotsuga menziesii): improving culture initiation with MES pH buffer, biotin, and folic acid. Plant Cell Tissue and Organ Culture 80: 91-103

Pullman, GS, Skryabina A (2007) Liquid medium and liquid overlays improve embryogenic tissue initiation in conifers. Plant Cell Report 26: 873-887

Ríos, L, Sánchez Olate M, Decarli N, Feito D, Rodríguez F (2005) Bases moleculares del enraizamiento. En: Sánchez-Olate M, Ríos D (Eds) Biotecnología vegetal en especies leñosas de interés forestal, pp 164. Departamento de Silvicultura. Facultad de Ciencias Forestales. Universidad de Concepción, Chile

Saborio, F, Dvorak William S, Donahue Jeffrey K, Thorpe Trevor A (1997) In vitro regeneration of plantlets from mature embryos of Pinus ayacahuite Ehrenb. Tree Physiology 17 (12): 787-796

Salanova, T, Blehova A, Salaj J (2007) Embryogenic suspension cultures of Pinus nigra Arn.: growth parameters and maturation ability. Acta Physiology Plant 29: 225-231

Schestibratov, K, Mikhailov R, Dolgov S (2003) Plantlet regeneration from subculturable nodular callus of Pinus radiata Don. Plant Cell Tissue and Organ Culture 72 (2): 139-146

Seelye, J, Burge G, Morgan E (2003) Acclimatizing Tissue Culture Plants: Reducing the Shock .New Zealand Institute for Crop & Food Research Limited. Combined Proceedings International Plant Propagators Society 53: 85-90

Selby, C, Kennedy S, Harvey B (1991) Adventitious root formation in hypocotyls cuttings of Picea sitchensis, the influence of plant growth regulators. New phytology 120: 453-457

Shenk, R, Hildebrandt A (1972) Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Canadian Journal of Botany 50: 199-204

Shim, SW, Hahn Ej, Paek Ky (2003) In vitro and ex vitro growth of grapevine rootstock `5BB' as influenced by number of air exchanges and the presence or absence of sucrose in culture media. Plant Cell, Tissue and Organ Culture 75: 57-62

Sinha, A, Hofmann M, Römer U, Köckenberger EL, Roitsch T (2002) Metabolizable and non-metabolizable sugars activate different signal transduction pathways in tomato. Plant Physiology 128: 1480-1489

Smeekens, S (2000) Sugar-induced signal transduction in plants. Annu. Rev. Plant Physiol. Plant Molecular Biology 51: 49-81

Stojicic, D, Budimir S (2004) Cytokinin-mediated axillary shoot formation in Pinus heldreichii. Biol Plant 48: 477-479

Stojicic, D, Budimir S, Culafic L (1999) Micropropagación of Pinus heldreichii. Plant Cell Tissue and Organ Culture 59: 147-150

Sul, W, Korban S (2004) Effects of salt formulation, carbon sources, cytokinins, and auxins on shoot organogenesis from cotyledons of Pinus pinea L. Plant Growth Regulation 43: 197-205

Tang, W, Guo Z (2001) In vitro propagation of loblolly pine via direct somatic organogenesis from mature cotyledons and hypocotyls. Plant Growth Regulation 33 (1): 25-31

Tang, W, Harris LC, Outhavong V, Newton RJ (2004) Antioxidants enhance in vitro plant regeneration by inhibiting the accumulation of peroxidase in Virginia pine (Pinus virginiana Mill.). Plant Cell Report 22: 871-877

Tang, W, Newton R, Charles TM (2006) Plant regeneration through multiple adventitious shoot differentiation from callus cultures of slash pine (Pinus elliottii Engelm.). Journal of Plant Physiology 163: 98-101

Tang, W, Newton RJ (2005) Plant regeneration from callus cultures derived from mature zygotic embryos in white pine (Pinus strobus L.). Plant Cell Report 24: 1-9

Tang, W, Ouyang F (2000) Plant regeneration via organogenesis from six families of loblolly pine. Plant Cell, Tissue and Organ Culture 58: 223-226

Tang, W, Ouyang F, Guo Z (1998) Plant regeneration through organogenesis from callus induced from mature zygotic embryos of loblolly pine. Plant Cell, Reports 17: 557-560

Thorpe, T, Stasolla C, Yeung EC, de Klerk GJ, Roberts A, George EF (2008) The Components of Plant Tissue Culture Media II : Organic Additions, Osmotic and pH Effects, and Support Systems. En: George, EF, Hall MA y de Klerk GJ (Eds) Plant Propagation by Tissue Culture 3rd Edition, pp. 115-173

Thorpe, TA (2004) Turning point article to root or not to root that is the question: reflections of a developmental plant physiologist. In Vitro Cellular Development Biology Plant 40: 128-142

Vales, T, Feng X, Ge L, Xu N, Cairney J, Pullman GS, Peter GF (2007) Improved somatic embryo maturation in loblolly pine by monitoring ABA-responsive gene expression. Plant Cell Report 26: 133-143

van Staden, J, Zazimalova E, George EF (2008) Plant Growth Regulators II: Cytokinins, their Analogues and Antagonists. En: George, EF, Hall MA y de Klerk GJ (Eds) Plant Propagation by Tissue Culture 3rd Edition, pp. 205-226. Springer. Dordrech

Warren, J, Roberts LW, Warren PM, Gresshoff PM (1994) Stimulatory and inhibitory effects of sucrose concentration on xylogenesis in lettuce pith explants; possible mediation by ethylene biosynthesis. Ann. Bot. 71: 65-73

Zhang, Y, Wei ZM, Xi ML, Shi JS (2006) Direct organogenesis and plantlet regeneration from mature zygotic embryos of masson pine (Pinus massoniana L.). Plant Cell Tissue and Organ Culture 84: 119-123

Zhu, LH, Wu XQ, Hong YQ, Jing J, Jian Y (2010) Micropropagation of Pinus massoniana and mycorrhiza formation in vitro. Plant Cell Tissue and Organ Culture 102: 121-128




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