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Análisis de la expresión de tres genes del metabolismo de los fenilpropanoides durante la interacción Musa spp.-Mycosphaerella fijiensis y en respuesta a etefón
Resumen
La ruta de los fenilpropanoides es una de las mejor estudiada, más manipulada y frecuentemente inducida por patógenos o elicitores de patógenos. Los perfiles de expresión de chalcona sintasa (CHS), similar a flavonoide 3’5' hidroxilasa (F3’5’H-like) y similar a la isoflavona reductasa (IFR-like), fueron analizados en plantas de ‘Calcutta 4’ (resistente) y ‘Grande naine’ (susceptible) a los 0, 6 y 12 días posteriores a la inoculación artificial con M. fijiensis y después del tratamiento con etefón 500 ì M. La técnica de reverso transcripción-reacción en cadena de la polimerasa (RT- PCR) con cebadores específicos, fue utilizada para este propósito. En presencia del hongo, los genes F3’5’H-like and IFR-like tuvieron expresión constitutiva mientras que el gen de la chalcona sintasa fue inducido (de una forma mas significativa en ‘Calcutta 4’), en el rango de tiempo probado para ambos genotipos. El productor de etileno etefón indujo el gen IFR-like en el genotipo resistente y el gen F3’5’H-like en el susceptible. Estos resultados permitirán continuar profundizando en el estudio del papel de los fenilpropanoides en la interacción Musa-M. fijiensis.
Palabras clave: chalcona sintasa (CHS), Musa spp., similar a flavonoide 3’5' hidroxilasa (F3’5’H-like), similar a la isoflavona reductasa (IFR-like)
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