Identificación y filogenia de la familia de proteínas tipo receptoras en tomate

Ermis Yanes-Paz, Gioser María Ramos-Echazábal, Glay Chinea, Yanelis Capdesuñer Ruiz, Ramón Santos Bermúdez

Resumen


Las proteínas tipo receptoras juegan múltiples papeles en procesos de desarrollo y defensa. En este trabajo se identificaron 75 RLPs en tomate (Solanum lycopersicum L.) mediante el empleo de búsquedas BLAST iterativas y predicción de dominios. Se construyó un árbol filogenético que incluyó todas las RLPs identificadas en tomate y otras proteínas de este tipo caracterizadas funcionalmente en otras especies. Primero se comprobó si la filogenia basada en la región C3-F constituía un buen indicador de la relación funcional entre proteínas relacionadas de diferentes especies. Se comprobó que, de hecho, las proteínas CLAVATA2 y su ortólogo en maíz FEA2 (FASCIATED EAR2) y la probable CLAVATA2 de tomate descrita en Uniprot se agruparon en una misma rama, lo que valida el enfoque. Con este procedimiento se identificó Solyc12g042760.1.1 como el probable homólogo en tomate del gen TMM. Se demostró que las proteínas en el mismo cluster en el árbol comparten relaciones funcionales, pues se formaron varios cluster de proteínas relacionadas funcionalmente como por ejemplo el cluster Ve, el cluster Cf, y la clada Eix.

 

Palabras clave: filogenia, receptores, RLP, tomate


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