Composición fitoquímica y actividad antibacteriana de extractos de hoja de Hamelia patens Jacq.

Yasmary Rubio Fontanills, Aymara L Valdivia Ávila, Conrado Camacho Campos, Madyu Matos Trujillo, Maryla Sosa del Castillo, Yunel Pérez Hernández

Resumen


El ponasí (Hamelia patens Jacq.) es un arbusto distribuido ampliamente en las regiones tropicales y de uso en la medicina tradicional para el tratamiento de heridas y procesos inflamatorios. El presente trabajo tuvo como objetivo determinar la composición fitoquímica y la actividad antibacteriana de extractos de hojas de ponasí presentes en áreas cercanas al Jardín Botánico de Matanzas, en la provincia de Matanzas, Cuba. Se colectaron hojas sanas en las cuales se determinaron las cantidades relativas de metabolitos secundarios y se cuantificó el contenido de fenoles solubles, ligados a pared celular y totales, así como de azúcares reductores. Se evaluó la actividad antimicrobiana del extracto etanólico frente a Staphylococcus aureus,Staphylococcus epidermidis y Escherichia coli. Se observó la presencia de terpenos, flavonoides, cumarinas, taninos, esteroides y glucósidos cardiotónicos en extractos metanólicos y etanólicos, que en el solvente etanólico estuvieron representados en mayores proporciones. El extracto etanólico de hoja tuvo un efecto antibacteriano frente a S. aureus y S. epidermidis, mientras que no mostró actividad inhibitoria frente a E. coli. El estudio fitoquímico y microbiológico realizado evidencia las potencialidades de Hamelia patens Jacq. como fuente de compuestos bioactivos con diferentes actividades biológicas y justifica el uso etnobotánico de esta especie en el tratamiento de infecciones bacterianas.

Palabras clave: fenoles, metabolitos secundarios, ponasí, Staphylococcus aureus


Texto completo:

PDF HTML

Referencias


Abubacker MN, Sathya C, Prabakaran R (2013) In vitro antifungal potentials of Hamelia patens Jacq. (Rubiaceae) aqueous extracts of leaves, flowers and fruits. Biosciences Biotechnology Research. Asia 10(2): 699-704; doi:10.13005/bbra/1183

Adamczyk B, Kitunen V, Smolander A (2013) Response of soil C and N transformations to condensed tannins and different organic N condensed tannin complexes. Applied Soil Ecology 64(5): 163-170; doi:10.1016/j.apsoil.2012.12.003

Ahmad A, Pandurangan A, Singh N, Ananad P (2012) A mini review on chemistry and biology of Hamelia patens (Rubiaceae). Pharmacognosy Journal 4(29): 1-4

Alonso-Castro AJ, Balleza-Ramos S, Hernandez-Morales A, Zapata-Morales JR, Gonzalez-Chavez MM, Carranza-Alvarez C (2015) Toxicity and antinociceptive effects of Hamelia patens. Revista Brasileira Farmacognosia 25(2): 170-176; doi:10.1016/j.bjp.2015.03.007

Basile A, Sorbo S, Spadaro V, Bruno M, Maggio A, Faraone N, Rosselli S (2009) Antimicrobial and antioxidant activities of Coumarins from the roots of Ferulago campestris (Apiaceae). Molecules 14(3): 939–952; doi:10.3390/molecules14030939

Cervantes L, Sánchez F, Gómez H (2017) Antibacterial activity of Cordia dentate Poir, Heliotropium indicum Linn and Momordica charantia Linn from the Northern Colombian Coast. Revista Colombiana de Ciencias Químicas y Farmacéuticas 46(2): 143-159; doi:10.15446/rcciquifa.v46n2.67933

Chahar S, Sharma J (2017) Phytochemical screening, total flavonoid and phenolic content assays and antioxidant activity of Momordica charantia L. leaves. Asian Journal of Pharmaceutical Education and Research 6(3): 60-69

Chigodi MO, Samoei DK, Muthangya M (2013) Phytochemical screening of Agave sisalana Perrine leaves (waste). International Journal of Applied Biology and Pharmaceutical Technology 4(4): 200-204

Cortés-Rojas DF, Chagas-Paula DA, Da Costa FB, Claudia R, Souza F, Oliveira WP (2013) Bioactive compounds in Bidens pilosa L. populations: a key step in the standardization of phytopharmaceutical preparations. Brazilian Journal of Pharmacognosy 23(1): 28-35; doi:10.1590/S0102-695X2012005000100

Cowan MM (1999) Plant products as antimicrobial agents. Clinical Microbiology Reviews 12(4): 564-582

Cushnie TP, Lamb AJ (2005) Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents 26(5): 343-56

Friend J (1992) Lignin and associated phenolic acids in cell walls. En: Gurr S J, McPherson M J, Bowles D J (eds). Molecular plant pathology: a practical approach, pp. 51- 59. IRC Press at Oxford University Press, Oxford

Gomez-Beloz A, Rucinski J C, Balick MJ, Tipton C (2003) Double incision wound healing bioassay using Hamelia patens from El Salvador. Journal of Ethnopharmacology 88(2): 169-173

Habu JB, Ibeh BO (2015) In vitro antioxidant capacity and free radical scavenging evaluation of active metabolite constituents of Newboul dialaevis ethanolic leaf extract. Biological Research 48(16): 1-10; doi:10.1186/s40659-015-0007-x

Jiménez-Suárez V, Nieto-Camacho A, Jiménez-Estrada M, Alvarado B (2016) Anti-inflammatory, free radical scavenging and alpha-glucosidase inhibitory activities of Hamelia patens and its chemical constituents. Pharmaceutical Biology 54(9): 1822-1830; doi:10.3109/13880209.2015.1129544

Jiménez-Suárez V, Reyes-Munguía A, Pérez-Berúmen C, Alvarado-Sánchez B (2012) Separación cromatográfica del extracto de Hamelia patens. Revista Académica de Investigación 11 (4): 1-10

Keerthana K, Deepa A, Shobana G, Jothi G, Sridharan G (2014) Preliminary phytochemical screening and in vitro antioxidant potential of Euphorbia heterophylla L.. International Journal of Pharmacy and Pharmaceutical Sciences 6(8): 550-553

Lawal OA, Amisu KO, Akinyemi SK, Sanni AA, Simelane MBC, Mosa RA, Opoku AR (2015) In vitro Antibacterial Activity of Aqueous Extracts of Bidens pilosa L. (Asteraceae) from Nigeria. British Microbiology Research Journal 8(4): 525-531; doi:10.9734/BMRJ/2015/17900

Madigan MT, Martinko JM, Bender K, Buckley S, Stahl DA (2015) Brock Biology of Microorganism. Pearson, Boston

Mesa-Vanegas AM, Zapata-Uribe S, Arana LM, Zapata IC, Monsalve Z, Rojano B (2015) Actividad antioxidante de extractos de diferente polaridad de Ageratum conyzoides L.. Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 14(1): 1–10

Miller G (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3): 426-428

MINSAP (1997) Guía metodológica para la investigación en plantas medicinales. Editorial Pueblo y Educación, La Habana

Mohammed MT, Abbas SI (2016) Antioxidant and Anti-Inflammatory Effect of Fruit Juice of Annona muricata L. (Soursop) During Ischemia Reperfusion Injury in Rats. The Iraqi Postgraduate Medical Journal 15(1): 118-123

Nayak BS, Ramdath DD, Marshall JR, Isitor GN, Eversley M, Xue S, Shi J (2010) Wound healing activity of the skin of the common grape (Vitis vinifera) Variant, Cabernet Sauvignon. Phytotherapy Research 24(8): 1151-1155; doi:10.1002/ptr.2999

Niranjan K, Sathiyaseelan V, Jeyaseelan EC (2013) Screening for anti-microbial and phytochemical properties of different solvents extracts of leafs of Pongamia pinnata. International Journal of Scientific and Research Publications 3(1): 1-3

Ojezele OJ, Ojezele MO, Adeosun AM (2016) Comparative phytochemistry and antioxidant activities of water and ethanol extract of Annona muricata Linn Leaf, seed and fruit. Advances in Biological Research 10(4): 230-235

Okoye EL, Ezeogo JI (2016) Antimicrobial Activity of the Crude Extracts of Hamelia patens on Some Selected Clinical Samples. Journal of Complementary and Alternative Medical Research 1(1): 1-7; doi:10.9734/JOCAMR/2016/27258

Omodamiro OD, Unekwe PC, Nweke IN, Jimoh MA (2014) Evaluation of diuretic activity of ethanol extract and its fractions of Agave sisalana. Journal of Pharmacy and Pharmaceutical Sciences 2(1): 1-6

Parekh J, Chanda S (2006) Antibacterial and phytochemical studies on twelve species of Indian medicinal plants. African Journal of Biomedical Research 10(2): 175- 181; doi:10.4314/ajbr.v10i2.50624

Patil A, Vadera K, Patil D, Phatak A, Juvekar A, Chandra N (2014) In vitro anticancer activity and phytochemical analysis of Bacopa monnieri (L.) Wettst. International Journal of Pharmacy Science Research 5(10): 4432-4438; doi:10.13040/IJPSR.0975-8232.5(10).4432-38

Pérez C, Paul M, Bazerque P (1990) An antibiotic assay by the agar well diffusion method. Acta Biologiae et Medicinae Experimentalis 15: 113-115

Pradhan D (2014) Pharmacological effect of some fractions obtained from Sapindus trifoliatus acting as an antioxidant and against mammary cell proliferation. African journal of pharmacy and pharmacology 8(17): 455-463; doi:10.5897/AJPP2014.4028

Quiñones M, Miguel M, Aleixandre A (2012) Los polifenoles, compuestos de origen natural con efectos saludables sobre el sistema cardiovascular. Nutrición Hospitalaria 27(1): 76-89

Rodríguez-García A, Martín JA, López R, Mutke S, Pinillos F, Gil L (2015) Influence of climate variables on resin yield and secretor structures in tapped Pinus pinaster Ait. in central Spain. Agricultural and Forest Meteorology 202(3): 83-93; doi:10.1016/j.agrformet.2014.11.023

Salzanoa S, Checconia P, Hanschmannc EM (2014) Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal. Proceeding of National Academy of Sciences of United States of America 111(33): 12157–12162; doi:10.1073/pnas.1401712111

Stojiljković D, Pavlović D, Arsić I (2014) Oxidative stress, sking aging and antioxidant therapy. Scientific Journal of the Faculty of Medicine in Nis 31(4): 207-217; doi:10.2478/afmnai-2014-0026

Surana AR, Kumbhare MR, Wagh RD (2016) Estimation of total phenolic and total flavonoid content and assessment of in vitro antioxidant activity of extracts of Hamelia patens Jacq. stems. Research Journal of Phytochemistry 10(2): 67-74; doi: 10.3923/rjphyto.2016.67.74

Surana A, Wagh R (2015) Phytopharmacological Review of Hamelia patens. International Journal for Pharmaceutical Research Scholars 4(2): 290-295

Usunomena U, Paulinus ON (2016) Phytochemical analysis and mineral composition of Annona muricata leaves. International Journal of Research and Current Development 1(1): 7-10

Varshney A, Shailajan S, Chandra N (2012) Estimation of Flavonoid-luteolin in different plant parts of Bacopa monnieri (L.) Wettst.by using HPTLC method. Analytical chemistry Indian Journal 11(1): 35-39

Vijayvergia R, Singh T, Sharma P, Khandelwal S (2011) Quantitative estimation and comparative study of primary metabolites of some medicinal plants. Current Pharmaceutical Research 2(5): 378-381

Vijayvergia R, Singh T, Sharma P, Khandelwal S (2012) Anthelmintic and antimicrobial activity of Hamelia patens Jacq. (Rubiaceae). International Journal of Natural Products Research 1(3): 54-56

Xie Y, Yang W, Tang F, Chen X, Ren L (2015) Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current Medicine Chemistry 22(1): 132-49

Yi J, Jian-Guo W, Yan-Bin W, Peng W (2016) Antioxidant and Anti-proliferative Activities of Flavonoids from Bidens pilosa L. var radiata Sch Bip. Tropical Journal of Pharmaceutical Research 15(2): 341-348; doi:10.4314/tjpr.v15i2.17

Zungu MM, Downs CT (2015) Effects of tannins on fruit selection in three southern African frugivorous birds. Behavioural processes 111: 84-89; doi:10.1016/j.beproc.2014.12.003




Copyright (c) 2018 Biotecnología Vegetal

Biotecnología Vegetal (https://revista.ibp.co.cu) ISSN 1609-1841, RNPS: 0397 (Versión impresa), ISSN 2074-8647, RNPS: 2154 (Versión electrónica) Editada por: Instituto de Biotecnología de las Plantas. Universidad Central Marta Abreu de Las Villas. Carretera a Camajuaní km 5.5, Santa Clara, Villa Clara, Cuba CP 54 830 Tel: 53 (42) 281257, 281268, 281693 Web: https://www.ibp.co.cu e-mail: info@ibp.co.cu          

Licencia de Creative Commons
Biotecnología vegetal está bajo una Licencia de Creative Commons Reconocimiento 4.0 Internacional.