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Factores que influyen en la transformación genética vía Agrobacterium tumefaciens en Phaseolus vulgaris L.
Resumen
El género Phaseolus, así como otras leguminosas, se ha mostrado recalcitrante a la regeneración de plantas in vitro y a la transformación genética vía Agrobacterium tumefaciens. La transferencia de genes en esta especie es limitada debido a su condición genotipo dependiente, además de resultar un proceso de baja eficiencia y poco reproducible. Existen evidencias que varios factores influyen en la integración del ADN y la transformación estable de plantas. Esta revisión tuvo como objetivo analizar la influencia de diferentes factores en la eficiencia de la transformación genética vía Agrobacterium tumefaciens en Phaseolus vulgaris L. tales como: cepa bacteriana, plasmidio, temperatura, condiciones de iluminación, tiempo de cocultivo, concentración bacteriana y marcador de selección.
Palabras clave: ADN, frijol común, leguminosas, plantas transgénicas.
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